大豆球蛋白A_(1b)B_2基因表达的分子基础探讨

通过对大豆品种松浦和A_(1b)B_2基因突变体凡实的染色体DNA进行限制性片段长度多态性(RFLP)分析,克隆了一个只存在于大豆品种松浦中,而不存在于大豆突变体凡实中的大小为2.2Kb的DNA片段,并利用pUC19为载体,对该片段进行了亚克隆,利用多种限制性内切酶进行了酶切分析,得到了该片段的酶切图谱。根据两个大豆品种在多种限制性内切酶酶切时都表现出显著差异,推断大豆球蛋白A(1b)B_2基因之所以不能在大豆品种凡实中表达,是由于该品种染色体DNA发生了倒位或缺失所致,而不仅仅是个别碱基对的突变。

AN INQUIRY INTO THE MOLECULAR BASES ABOUT SOYBEAN GLYCININ A__1bB_2 GENE EXPRESSION

DNA restriction fragment length polymorphism (RFLP) analysis was carried out using a labelled soybean glycinin A1bB2 cDNA as probe. The seedlings of soybean cultivar Matsuura and variety mutant Bonminori were used as experiment materials. One 2. 2Kb DNA fragmemt which exists only in cultivar Matsuura but not in variety mutant Bonminori was cloned into Charomid 9 - 42. Then it was subcloned using pUC19 as vector. The restriction map of that 2. 2Kb DNA fragment was made according to the results of several restriction enzyme digestions. It was deduced that the products of soybean glycinin A1bB2 gene expression which can not be detected in variety mutant Bonminori is probably due to the rearrangement (inversion) or deletion. It is not the mutation of a few base pairs.