花生种子白藜芦醇含量QTL定位分析

本研究利用“中花10×ICG 12625”衍生的RIL群体共140个家系及其亲本为材料，通过高效液相色谱检测各家系在2014年及2015年收获的花生种子中的白藜芦醇含量。结果表明，RIL群体白藜芦醇含量变异范围为38.27~352.08 μg/ kg。通过两年重复检测，获得稳定高白藜芦醇含量材料4份(QT0339、QT0369、QT0450和QT0454)，含量为143.90~215.60 μg/ kg，在2014年环境中，这四份材料白藜芦醇含量分别超过高值亲本32.31 %、5.80 %、86.46 %和79.52 %，在2015年环境中白藜芦醇含量分别超过高值亲本106.04 %、49.78 %、7.90%和52.87%。利用前期通过该群体构建的遗传连锁图谱，应用WinQTLCart 2.5软件定位到花生种子白藜芦醇含量相关QTL13个，贡献率在2.2 %~7.4 %之间，其中qRB8.1a以及qRB8.1b为两年环境中重复检测到的QTL。本文研究结果为培育白藜芦醇含量高的花生新品种提供理论依据。

QTL Analysis of Resveratrol Content in Peanut Seeds

 Resveratrol is an important component of phytoalexins, and it affects human cardiovascular system. In this study, the resveratrol content in seeds of 140 lines in an RIL population (Zhonghua 10 × ICG 12625) was detected by high performance liquid chromatography (HPLC) in two environments. The result showed that the variation resveratrol contents in the RIL population ranged from 38.27 μg/ kg to 352.08 μg/ kg. Four Lines (QT0339, QT0369, QT0450 and QT0454) showed stable higher resveratrol contents than the high value parent in the two environments, ranging from 143.90 μg/ kg to 215.60 μg/ kg. The resveratrol contents of these lines were higher than that of the high value parent by 32.31 %, 5.80 %, 86.46% and 79.52 % in 2014 environment and 32.31 %, 5.80 %, 86.46 % and 79.52 % in 2015 environment, respectively. A total of 13 QTLs related to resveratrol content in peanut seeds were detected by WinQTLCart 2.5 software, and showed phenotypic variations of 2.2-7.4%. Notably, qRB8.1a and qRB8.1b were repeatedly detected in both environments. The results of this study provided a theoretical basis for breeding new peanut varieties with high resveratrol content.