| 风箱树组织培养研究 |
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| 引用本文： | 马妍,朱建峰,赵健如,王进茂.风箱树组织培养研究[J].河北林果研究,2012,27(2):147-151. |
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| 作者姓名： | 马妍 朱建峰 赵健如 王进茂 |
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| 作者单位： | 1. 河北农业大学林学院,河北保定,071000
2. 中国林业科学研究院林业研究所,北京,100091 |
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| 基金项目： | 科技部"十一五"科技支撑计划，国家林业局科技推广项目 |
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| 摘 要： | 以风箱树(Cephalanthus occidentalis L.)带腋芽的茎段为外植体,探讨不同植物激素(6-BA、IBA、NAA)对试管苗增殖以及植株再生的影响,建立了一套风箱树离体培养技术体系。试验结果表明:启动最适培养基为MS0;增殖最适培养基为MS+6-BA 1.5mg/L+IBA 0.2mg/L;生根最适培养基为1/4MS0;诱导率可达73.3%,增殖系数可达3.55,生根率89.47%,移栽成活率可达到100%。
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| 关 键 词： | 风箱树 组织培养 快速繁殖 |
Tissue culture of Cephalanthus occidentalis L. |
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| MA Yan
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ZHU Jian-feng
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ZHAO Jian-ru
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WANG Jin-mao.Tissue culture of Cephalanthus occidentalis L.[J].Hebei Journal of Forestry and Orchard Research,2012,27(2):147-151. |
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| Authors: | MA Yan ZHU Jian-feng ZHAO Jian-ru WANG Jin-mao |
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| Institution: | 1 Forestry College, Agricultural University of Hebei , Baoding 071000,China ; 2 Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China) |
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| Abstract: | Stems with axillaries bud of Cephalanthus occidentalis L. were used as explants and the effects of different plant auxins （6 -BA, NAA, IBA） on proliferation and regenera-tion of Cephalanthus occidentalis L. were studied. The results showed that the suitable me-dium for bud germination was MS0 ; the optimum multiplication culture medium was MS＋6 - BA 1.5 mg/L＋IBA 0.2 mg/L; and the rooting culture medium was 1/4MS0. In this cultivation system, the rate of induction was 73.3%, with the highest propagation coefficient of 3.55, 89.47% rooting rate and 100% survival rate of transplant. |
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| Keywords: | Cephalanthus occidentalis L tissue culture rapid propagation |
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