| 一种适于番茄RAPD分析的DNA快速提取方法 |
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| 引用本文： | 杨瑞,赵福宽,林成,高遐虹,程继鸿. 一种适于番茄RAPD分析的DNA快速提取方法[J]. 北京农学院学报, 2003, 18(4): 278-280 |
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| 作者姓名： | 杨瑞 赵福宽 林成 高遐虹 程继鸿 |
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| 作者单位： | 北京农学院生物技术系,北京,102206;新疆农业大学园艺学院,乌鲁木齐,830052;北京农学院植物科学技术系,北京,102206 |
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| 基金项目： | 北京市科技新星计划项目;953811100; |
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| 摘 要： | 笔者通过比较快速提取方法和常规方法提取的DNA的质量和RAPD扩增结果,建立了一种适合番茄RAPD分析的快速,简单,成本低的DNA提取方法。该方法的材料不用液氮和Rnase消化处理。
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| 关 键 词： | 番茄 DNA快速提取 RAPD |
| 文章编号： | 1002-3186(2003)04-0278-03 |
| 修稿时间： | 2003-08-08 |
A Simple and Rapid Method of Tomato Genomic DNA Extraction for RAPD Analysis |
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| YANG Rui,ZHAO Fu-Kuan,LIN Cheng,GAO Xia-Hong,CHEN Ji-Hong. A Simple and Rapid Method of Tomato Genomic DNA Extraction for RAPD Analysis[J]. Journal of Beijing Agricultural College, 2003, 18(4): 278-280 |
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| Authors: | YANG Rui ZHAO Fu-Kuan LIN Cheng GAO Xia-Hong CHEN Ji-Hong |
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| Affiliation: | YANG Rui~1,ZHAO Fu-Kuan~1,LIN Cheng~2,GAO Xia-Hong,CHEN Ji-Hong~3 |
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| Abstract: | Based on experiments , comparison of quality and RAPD results of tomato genomic DNA with two extraction methods, common and fast method which is a simple , rapid and low cost was established for DNA extraction from tomato younger leaves .It was especially suitable for RAPD analysis since that the materials needn't to be treated with liquid nitrogen and Rnase. |
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| Keywords: | tomato DNA rapid extraction RAPD analysis |
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