| 放养型和野生型柞蚕的核糖体基因转录间隔区Ⅱ(ITS-2)的克隆与序列比较 |
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| 引用本文： | 王振东,武松,曲泽岚,齐永红,朱绪伟,李喜升,秦利,刘彦群.放养型和野生型柞蚕的核糖体基因转录间隔区Ⅱ(ITS-2)的克隆与序列比较[J].蚕业科学,2010,36(1):60-65. |
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| 作者姓名： | 王振东 武松 曲泽岚 齐永红 朱绪伟 李喜升 秦利 刘彦群 |
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| 作者单位： | 沈阳农业大学生物科学技术学院,沈阳,110161;山西省果业工作总站,太原,030001;河南省蚕业科学研究院,河南云阳,474676;辽宁省蚕业科学研究所,辽宁凤城,118100 |
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| 基金项目： | 国家自然科学基金项目(No.30800803);;公益性行业(农业)科研专项(No.nyhyzx07-020-17);;现代农业产业技术体系建设专项(蚕桑) |
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| 摘 要： | 核糖体基因转录间隔区Ⅱ(ITS-2)在物种间甚至种内群体间均表现出较高的差异,被用于种内或近缘种的研究。测定了放养型和野生型柞蚕(Antheraea pernyi)的rDNA ITS-2全长序列(GenBank登录号:GU073314、GU073315),并分析了二者之间的遗传差异。放养型和野生型柞蚕的rDNA ITS-2全长分别为1 111、1 112 bp,2个序列的GC含量达到61%,相似性高达98%。序列比对后得到1 117 bp的对齐序列,共鉴定变异位点19个,其中转换位点8个,无颠换,有11处发生核苷酸的插入/缺失。基于K2P模型计算的2条rDNA ITS-2序列的遗传距离为0.007,这一数值与估算的鳞翅目昆虫rDNA ITS-2序列种内平均遗传距离(0.009)基本一致。
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| 关 键 词： | 柞蚕 放养型 野生型 核糖体基因转录间隔区Ⅱ 遗传变异 |
Cloning and Sequence Comparison of rDNA Internal Transcribed Spacer 2 (ITS-2) from the Domestic and Wild Type of Chinese Oak Silkworm, Antheraea pernyi |
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| WANG Zhen-Dong,WU Song,QU Ze-Lan,QI Yong-Hong,ZHU Xu-Wei,LI Xi-Sheng,QIN Li,LIU Yan-Qun.Cloning and Sequence Comparison of rDNA Internal Transcribed Spacer 2 (ITS-2) from the Domestic and Wild Type of Chinese Oak Silkworm, Antheraea pernyi[J].Acta Sericologica Sinica,2010,36(1):60-65. |
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| Authors: | WANG Zhen-Dong WU Song QU Ze-Lan QI Yong-Hong ZHU Xu-Wei LI Xi-Sheng QIN Li LIU Yan-Qun |
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| Institution: | 1College of Bioscience and Biotechnology;Shenyang Agricultural University;Shenyang 110161;China;2 Shanxi Fruit Industry General Station;Taiyuan 030001;3Henan Sericultural Research Institute;Yunyang Henan 474676;4Liaoning Sericultural Institute;Fengcheng Liaoning 118110;China |
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| Abstract: | The ribosomal internal transcribed spacer sequences 2(ITS-2) are highly divergent between species or even between various populations of the same species.Therefore,they are often used in studies on intraspecies or their close relatives.In the present study,full-length rDNA sequences from the domestic and wild type of Chinese oak silkworm,Antheraea pernyi(GenBank No.GU073314 and GU073315,respectively) were obtained and comparatively analyzed on their genetic variation.Sequence analysis revealed that the whol... |
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| Keywords: | Antheraea pernyi Domestic type Wild type Ribosomal internal transcribed spacer 2 Genetic variation |
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