契斯曼尼番茄果实中可溶性固形物和β–胡萝卜素含量相关基因QTL 分析

 以野生番茄契斯曼尼LA0317与栽培番茄自交系9706为亲本，构建了包括152个株系的BC₂S₆群体。利用155个SSR标记、21个CAPS标记和88个Indel标记构建了契斯曼尼番茄的遗传连锁图谱，总图距1 134.0 cM，标记间平均距离4.29 cM。采用复合QTL区间作图法对番茄果实中的可溶性固形物含量和β–胡萝卜素含量进行QTL分析。定位到10个与可溶性固形物含量相关的QTLs，分别位于第3、4、5、6、9、10、11号染色体上，其中有5个QTL效应值在12%以上。定位到6个与β–胡萝卜素含量相关的QTLs,分别位于第1、2、6、9号染色体上。其中,位于6号染色体上的qBC6b效应值达到54.7%，为一主效QTL。此外，还获得了可用作育种材料的5个高可溶性固形物含量株系和8个高β–胡萝卜素含量的株系。

QTL Mapping of Soluble Solids Content and Beta-carotene in Solanum cheesmanii

A population BC₂S₆ with 152 lines was developed from the cross between Solanum lycopersicum 9706 and Solanum cheesmanii LA0317. A linkage map was constructed with 155 SSR marker，21 CAPS marker and 88 Indel marker. The map covered 1 134.0 cM，and the average distance between adjacent loci was 4.29 cM．Using MQM-mapping method，QTL of soluble solids content（SSC）and Beta-carotene content were detected. Ten QTL of SSC were mapped to chromosome 3，4，5，6，9，10 and 11. Respectively，five of which explained more than 12% of the phenotypic variation. Six QTL of Beta-carotene content were mapped to chromosome 1，2，6，9. The major QTL of Beta-carotene content on chromosome 6 could explain 54.7% of the phenotypic variation．Moreover，5 lines with high SSC and 8 lines with high Beta-carotene content were obtained，and could be used as breeding materials.