山楂多酚类物质的提取及其抗氧化活性研究

目的]研究山楂中多酚类物质的提取和体外抗氧化作用,为山楂作为抗氧化、抗衰老的保健食品的开发提供理论依据。方法]通过70%乙醇提取与D101大孔吸附树脂纯化制备山楂多酚;采用铁离子还原/抗氧化能力测定法、邻苯三酚自氧化法、二苯代苦味酰基自由基(DPPH.)分析法及油脂过氧化值测定法,测定山楂多酚的体外抗氧化能力。结果]纯化制备的山楂多酚含量为60.24%;山楂多酚对羟自由基(.OH)的50%清除率(IC50)为53.4μg/ml;对O2-.的50%清除率(IC50)为42.3μg/ml;对DPPH.的50%清除率(IC50)为63.3μg/ml;山楂多酚有较强的抗猪油氧化作用,能显著抑制猪油POV的增加。结论]山楂多酚的提取工艺比较简单,有较强的体外抗氧化能力。

Extraction Technology of Polyphenols in Crataegus pinnatifida and Its Antioxidant Activity

Objective] To study the extraction of in vitro antioxidant activity of polyphenols from Crataegus pinnatifida,so as to provide theoretical basis for the development of antioxidant and anti-aging products.Method] The polyphenols were firstly extracted with 70% ethanol and then purified by macroporous adsorption resin.Their in vitro antioxidant activity was studied by iron ion reduction/oxidation resistance measurement,adjacent benzene three phenol from oxidation,two benzene and bitter acyl free radicals analysis method and oil peroxide value measurement.Result] 60.24% Crataegus pinnatifida polyphenols were prepared,their 50% scavenging capacity(IC50) of ·OH,O2-· and DPPH· was 53.4,42.3 and 63.3 μg/ml,the polyphenols can significantly inhibit the antioxidation and POV increase of lard oil.Conclusion] The extraction technology of polyphenols is simple and the extracted polyphenols showed strong in vitro antioxidant activity.