超临界CO2萃取法与水蒸气蒸馏法提取香茅草挥发油化学成分比较

目的]比较不同方法提取的香茅草挥发油化学成分。方法]采用超临界CO2流体萃取法(SCDE)及水蒸气蒸馏法(SD)从香茅草中提取挥发油,用气相色谱-质谱联用技术(GC-MS)对其化学成分进行定性定量分析。结果]在超临界CO2流体萃取法提取的挥发油中共鉴定了31种成分,占挥发油总成分的91%以上;在水蒸气蒸馏法提取的挥发油中共鉴定了17种成分,占挥发油的94%以上。2种提取方法得到的挥发油组分及其含量差异较大,含量最高的都是香叶醛和橙花醛。结论]超临界CO2流体萃取法提取的挥发油比水蒸气蒸馏法能更真实、全面的反映药材中的化学成分。

Comparison of Chemical Components in the Essential Oil Extracted by Supercritical CO2 Fluid and Steam Distillation from Cymbopogon citratus

Objective] To compare the chemical components in the essential oil extracted by different methods from Cymbopogon citratus(DC.) Stapf.Method] The essential oils were extracted by supercritical CO2 fluid and steam distillation.And then their chemical components were qualitatively and quantitatively analyzed by GC-MS.Result] 31 components were identified in the essential oil extracted by supercritical CO2 fluid from Cyrnbopogon citratus,accounting for over 91% of the total volatile components;while 17 components were identified in the essential oil extracted by steam distillation,accounting for about 94% of the total components.The essential oil extracted by the two methods had different types and different contents of chemical components,geranial and neral were the most.Conclusion] The essential oil extracted by supercritical CO2 fluid is better than that extracted by steam distillation to truly and comprehensively reflect the chemical components in medicines.