萱草再生体系技术建立的研究 |
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引用本文: | 文伟,杨骥.萱草再生体系技术建立的研究[J].中国园艺文摘,2010,26(3):8-10. |
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作者姓名: | 文伟 杨骥 |
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作者单位: | 铁岭师范高等专科学校,辽宁,铁岭,112001 |
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摘 要: | 以萱草的根茎为材料,进行愈伤组织诱导和分化,试管苗的生根、移栽和移植的研究,建立萱草再生体系技术。结果证明:MS+BA0.4mg/L+NAA0.1mg/L+2,4-D0.1mg/L是愈伤组织诱导培养的理想培养基;MS+NH_4H_2PO_450mg/L+BA1.0mg/L+NAA0.1mg/L是愈伤组织增殖继代培养的理想培养基;MS+AgNO_30.5mg/L+GA_30.5mg/L+BA0.5mg/L+NAA0.1mg/L是愈伤组织分化培养的理想培养基;1/2MS+NAA0.1mg/L+IAA0.2mg/L是不定芽生根培养的理想培养基。在河沙中试管苗易移栽成活;移植到花坛中的试管苗根系发达、生长旺盛。
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关 键 词: | 萱草 组织培养 再生体系 |
Establishment of Regeneration System of Hemerocallis fulva |
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Authors: | WEN Wei YANG Ji |
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Institution: | WEN Wei YANG Ji |
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Abstract: | The study was mainly to probe an efficient way through tissues culture and rapid propagation of Hemerocallis fulva. The rhizome was taken as explants.The main results are as follows:The optimum medium was MS+BA0.4mg/L+NAA0.1mg/L+2,4-D0.1mg/L for inducing callus; the optimum culture medium of proliferating callus was MS+NH4H2PO450mg/L+BA1.0mg/L+NAA0.1mg/L; the optimum culture medium of differentiating callus was MS+AgNO30.5mg/L+GA30.5mg/L+BA 0.5mg/L+NAA 0.1mg/L; the optimum culture medium for rooting was 1/2MS+NAA0.1mg/L+IAA0.2mg/L.The transplant medium with riversand is optimal for Hemerocallis fulva. Seedling transferred from test tubes grew well. |
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Keywords: | Hemerocallis fulva tissue culture regeneration system |
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