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| 酿酒酵母耐铜基因CUP1的克隆及其植物表达载体的构建 | |
| 引用本文： | 何勇强,陶勤南,唐纪良,臧宁,小繧仁. 酿酒酵母耐铜基因CUP1的克隆及其植物表达载体的构建[J]. 广西农业生物科学, 2000, 19(4): 233-238 |
| 作者姓名： | 何勇强 陶勤南 唐纪良 臧宁 小繧仁 |
| 作者单位： | 1. 广西大学生物技术实验中心,南宁,530005 2. 浙江大学环境与资源学院,杭州,310029 3. 日本三重大学生物资源学部,日本三重 514-8507 |
| 基金项目： | 中国科学院资助项目，广西自然科学基金，398600038，9912002，， |
| 摘 要： | 酿酒酵母铜金属硫蛋白（ｃｏｐｐｅｒ ｍｅｔａｌｌｏｔｈｉｏｎｅｉｎ，Ｃｕ－ＭＴ），属于第Ⅱ型金属硫蛋白，Ｃｕ－ＭＴ由酿酒酵母耐铜基因ＣＵＰ１编码，由６１个氨基酸残基组成，其中半胱氨酸残基１３个，占Ｃｕ－ＭＴ总氨基酸数的２１．３％，Ｃｕ－ＭＴ与铜离子有较强的结合力，在酵母细胞中主要参与过量铜的解毒作用。本研究通过ＰＣＲ方法，从酵母基因组ＤＮＡ中扩增出了ＣＵＰ１基因，克隆于ｐＵＣ１９的多克隆拉点。扩增 |
| 关 键 词： | 植物表达载体 CUP1基因 克隆 土壤 重金属污染 |
| 修稿时间： | 1999-09-29 |
Cloning of yeast CUP1 gene and construction its plant expression vector |
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| HE Yong-qiang,TANG Ji-liang,ZANG Ning,TAO Qin-nan,Hitoshi Obata. Cloning of yeast CUP1 gene and construction its plant expression vector[J]. Journal of Guangxi Agricultural and Biological Science, 2000, 19(4): 233-238 | |
| Authors: | HE Yong-qiang TANG Ji-liang ZANG Ning TAO Qin-nan Hitoshi Obata |
| Abstract: | The baker yeast copper metallothionein (CuMT), the class II metallothionein, consists of 61 amino acid residues, including 13 cysteine residues. CuMT was encoded by yeast CUP1 gene. Possessing special affinity to copper ions, CuMT plays a key role in copper detoxification in yeast cells. In this study, the CUP1 gene was amplified from baker yeast genomic DNA by PCR and cloned into the MCS of pUC19. The amplified fragment was 486 bp, including ORF of CUP1 and part of its downstream sequence. DNA sequence analysis indicated that the ORF of the amplified fragment was same as the published ORF of CUP1 and the amino acid sequence of the putative polypeptide was the same as that of CUP1 .The plant expression vectors, named pBICUP1 and pCMCUP1 respectively, have been constructed for transformation of rice, ramie and hydrophytes. |
| Keywords: | CUP1 gene copper tolerance plant expression vecP |
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