| 尖孢镰刀菌古巴专化型4号生理小种红色荧光蛋白基因转化 |
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| 引用本文： | 谢德啸,羊玉花,周端咏,郭立佳,彭军,黄小娟,齐兴柱,黄俊生.尖孢镰刀菌古巴专化型4号生理小种红色荧光蛋白基因转化[J].热带作物学报,2012,33(4):685-689. |
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| 作者姓名： | 谢德啸 羊玉花 周端咏 郭立佳 彭军 黄小娟 齐兴柱 黄俊生 |
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| 作者单位： | 1 海南大学农学院
2 中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室;1 海南大学农学院
2 中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室;1 海南大学农学院
2 中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室;中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室 |
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| 基金项目： | 国家公益性行业（农业）科研专项“由尖孢镰刀菌引起的作物土传病害综合防控技术研究”（No. 200903049）；中央级公益性科研院所基本科研业务费专项（No. 2011HZS1J022）。 |
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| 摘 要： | 采用PEG-CaCl2介导的原生质体转化体系，成功地将红色荧光蛋白基因DsRed转入到尖孢镰刀菌古巴专化型4号生理小种B2菌株中。在显微镜下观察连续转接6代的转化子，结果都可以观察到很强的红色荧光，表明DsRed基因在转化的B2菌株中能稳定遗传。通过PCR验证，也表明红色荧光蛋白基因DsRed已经转入到尖孢镰刀菌中。对香蕉的致病力测定结果表明，转化子与野生型菌株B2相比没有明显差异。
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| 关 键 词： | 尖孢镰刀菌古巴专化型 DsRed 原生质体转化 致病力 香蕉枯萎病 |
Red Fluorescent Protein Gene(DsRed) Transformation of Fusarium oxysporum f. sp. cubense race 4 |
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| XIE Dexiao,YANG Yuhu,ZHOU Duanyong,GUO Liji,PENG Jun,HUANG Xiaojuan,QI Xingzhu and HUANG Junsheng.Red Fluorescent Protein Gene(DsRed) Transformation of Fusarium oxysporum f. sp. cubense race 4[J].Chinese Journal of Tropical Crops,2012,33(4):685-689. |
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| Authors: | XIE Dexiao YANG Yuhu ZHOU Duanyong GUO Liji PENG Jun HUANG Xiaojuan QI Xingzhu and HUANG Junsheng |
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| Institution: | 1 College of Agronomy, Hainan University
2 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science / Key Laboratory of Monitoring and
Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agri;1 College of Agronomy, Hainan University
2 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science / Key Laboratory of Monitoring and
Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agri;1 College of Agronomy, Hainan University
2 Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science / Key Laboratory of Monitoring and
Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agri;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science / Key Laboratory of Monitoring and
Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science / Key Laboratory of Monitoring and
Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science / Key Laboratory of Monitoring and
Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science / Key Laboratory of Monitoring and
Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science / Key Laboratory of Monitoring and
Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture |
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| Abstract: | The report gene coding red fluorescent protein(DsRed)was successfully transferred to the B2 strain of Fusarium oxysporum f. sp. cubense race 4(Foc4), by the protoplast-mediated genetic transformation system. After six successive subculture, the strong red fluorecence could be observed in the obtained transformants under fluorescence microscope. The PCR amplification targeted the DsRed gene confirmed that the DsRed gene had been transferred to the B2 strains of Foc4. Additionally, the pathogenecity of the transformants didn't vary compared with the wild type. In conclusion, the trsanformants with the DsRed gene would do us a favor to observe the pathogenesis of Foc4 in banana plants. |
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| Keywords: | Fusarium oxysporum f sp cubense DsRed Protoplast-mediated genetic transformation system Fusarium wilt of banana |
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