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| 辣椒环斑病毒分子检测方法的建立及应用 | |
| 引用本文： | 王健华,章绍延,龚殿,张雨良,刘志昕. 辣椒环斑病毒分子检测方法的建立及应用[J]. 热带作物学报, 2012, 33(2): 342-345 |
| 作者姓名： | 王健华 章绍延 龚殿 张雨良 刘志昕 |
| 作者单位： | 1. 海南大学环境与植物保护学院,海南儋州571737;中国热带农业科学院热带生物技术研究所 农业部热带作物生物技术重点开放实验,海南海口571101 2. 中国热带农业科学院热带生物技术研究所 农业部热带作物生物技术重点开放实验,海南海口,571101 |
| 基金项目： | 海南大学“211工程”建设项目；中央级公益性科研院所基本科研业务费（No.ITBB110304）。 |
| 摘 要： | 辣椒环斑病毒（Chilli ringspot virus， ChiRSV）是2007年在辣椒上发现的病毒新种，是马铃薯Y病毒属（Potyvirus）的确定种。笔者于2009年首次在海南黄灯笼辣椒上检测发现ChiRSV。根据ChiRSV保守区域设计和筛选PCR特异引物，优化退火温度，对PCR产物进行序列测定，建立了ChiRSV的RT-PCR检测方法。通过灵敏度测定，结果表明该方法最低可检出12.5 pg病毒RNA，灵敏度高。通过对来自海南田间的疑似辣椒病样的RT-PCR检测，证实了该方法有良好的反应特异性，亦表明ChiRSV可能已在海南扩散。 |
| 关 键 词： | 辣椒环斑病毒；分子检测；黄灯笼辣椒 |
Establishment and Application for Molecular Detection of Chilli ringspot Virus |
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| WANG Jianhu,ZHANG Shaoyan,GONG Dian,ZHANG Yuliang and LIU Zhixin. Establishment and Application for Molecular Detection of Chilli ringspot Virus[J]. Chinese Journal of Tropical Crops, 2012, 33(2): 342-345 | |
| Authors: | WANG Jianhu ZHANG Shaoyan GONG Dian ZHANG Yuliang LIU Zhixin |
| Affiliation: | 1 College of Environmemt and Plant Protection, Hainan University2 Key Laboratory for Tropical Crop Biotechnology, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, CATAS,;1 College of Environmemt and Plant Protection, Hainan University2 Key Laboratory for Tropical Crop Biotechnology, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, CATAS,;1 College of Environmemt and Plant Protection, Hainan University2 Key Laboratory for Tropical Crop Biotechnology, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, CATAS,;Key Laboratory for Tropical Crop Biotechnology, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, CATAS,;Key Laboratory for Tropical Crop Biotechnology, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, CATAS, |
| Abstract: | Chilli ringspot virus(ChiRSV)is a novel virus discovered in 2007 in chili pepper.ChiRSV was first detected on Capsicum chinense Jacp.Cv.Yellow Lantern in Hainan province in 2009 by the authors.The RT-PCR detection method was established in this research,which including specific primers designing,annealing temperature testing.This method showed good amplification reaction,and the sensitivity was as high as 12.5 pg virus RNA.RT-PCR detection of field samples demonstrated the sensitivity and specificity of the method in this paper,indicating the possibility of the spread of ChiRSV in Hainan Province. |
| Keywords: | Chilli ringspot virus Molecular detection Capsicum chinense |
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