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| 桔小实蝇抗菌肽Bactrocerin-2的分离纯化与鉴定 | |
| 引用本文： | 党向利,田金环,易辉玉,柴一秋. 桔小实蝇抗菌肽Bactrocerin-2的分离纯化与鉴定[J]. 热带作物学报, 2012, 33(3): 556-561 |
| 作者姓名： | 党向利 田金环 易辉玉 柴一秋 |
| 作者单位： | 1. 浙江省亚热带作物研究所 温州市虫生真菌资源研究与开发重点实验室 浙江温州325005 2. 暨南大学材料科学与工程系,广东广州,510632 3. 华南农业大学动物科学学院,广东广州,510642 |
| 基金项目： | 浙江省自然科学基金(No. Y3110597)；广东省自然科学基金(No. 032256)。 |
| 摘 要： | 采用阳离子交换层析、RP-FPLC及RP-HPLC分离纯化技术，从细菌诱导的桔小实蝇蛹中分离鉴定出桔小实蝇抗菌肽Bactrocerin-1的同系物Bactrocerin-2。结果表明：Bactrocerin-2含有20个氨基酸残基，分子量为2 311.51 u，序列为VTKTWIKVIRGIGKSKIKWA；并且Bactrocerin-2与Bactrocerin-1的相似性极高，氨基酸同源性达到90％；该肽与鞘翅目Coleoptericin C-末端的20个氨基酸也具有较高的相似性。氨基酸组成分析结果表明，该肽是一种疏水、带正电荷的抗菌肽。该研究将有助于对昆虫天然免疫反应的认知，并为设计有效的抗菌分子奠定基础。 |
| 关 键 词： | 桔小实蝇；抗菌肽；Bactrocerin-2；同系物；双亲性 |
Purification and Characterization of Bactrocerin-2 from Oriental Fruit fly Bactrocera dorsalis Hendel |
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| DANG Xiangli,TIAN Jinhuan,YI Huiyu and CHAI Yiqiu. Purification and Characterization of Bactrocerin-2 from Oriental Fruit fly Bactrocera dorsalis Hendel[J]. Chinese Journal of Tropical Crops, 2012, 33(3): 556-561 | |
| Authors: | DANG Xiangli TIAN Jinhuan YI Huiyu CHAI Yiqiu |
| Affiliation: | Zhejiang Institute of Subtropical Crops/ Key Laboratory of Entomogenous Fungi Resources Research and Development of Wenzhou;Department of Materials Science and Engineering, Jinan University;College of Animal Science, South China Agricultural University;Zhejiang Institute of Subtropical Crops/ Key Laboratory of Entomogenous Fungi Resources Research and Development of Wenzhou |
| Abstract: | Bactrocerin-2(20-residues)was purified from the immunized pupae of oriental fruit fly Bactrocera dorsalis Hendel by cation-exchange chromatography,RP-FPLC and RP-HPLC.It was an isoform of Bactrocerin-1.Molecular mass of Bactrocerin-2 was determined as 2 311.51 u by MALDI-TOF-MS.Complete amino acid sequences was VTKTWIKVIRGIGKSKIKWA.It showed very high similarity to C-terminal sequences of Coleoptericin.The composition of amino acid residues revealed that Bactrocerin-2 was a hydrophobic,positively charged peptide. |
| Keywords: | Bactrocera dorsalis Antimicrobial peptide Bactrocerin-2 Isoform Amphipathicity |
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