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铁观音与荆芥提取物协同抗氧化作用研究
引用本文:庞海月,黄立森,陈煜沛,吴黉坦,陈芳芳,王贵弘.铁观音与荆芥提取物协同抗氧化作用研究[J].中国茶叶,2021(1):50-53.
作者姓名:庞海月  黄立森  陈煜沛  吴黉坦  陈芳芳  王贵弘
作者单位:厦门医学院公共卫生与医学技术系;天然化妆品福建省高校工程研究中心
基金项目:天然化妆品福建省高校工程研究中心资助。
摘    要:为研究铁观音提取物和荆芥提取物联用在抗氧化方面的协同作用,采用水浸提法分别萃取铁观音和荆芥得其抗氧化活性成分。以芦丁为标准品检测两种提取物类黄酮含量,其中铁观音提取物为15.13 mg/g,荆芥提取物为0.87 mg/g;以没食子酸为标准品检测两种提取物的总酚含量,其中铁观音水提物为50.87μg/mg,荆芥水提物为47.40μg/mg。用2,2-二苯基-1-苦基肼(DPPH)和2,2'-二氮-双(3-乙基苯并噻唑-6-磺酸)(ABTS)法评估两种提取物联合应用时的抗氧化活性,结果表明,在较低浓度时,铁观音与荆芥提取物联用具有协同增强抗氧化作用。

关 键 词:协同作用  抗氧化  2  2-二苯基-1-苦基肼  2  2'-二氮-双(3-乙基苯并噻唑-6-磺酸)

Study on the Synergistic Antioxidant Effect of Tieguanyin and Schizonepeta Extracts
PANG Haiyue,HUANG Lisen,CHEN Yupei,WU Hongtan,CHEN Fangfang,WANG Guihong.Study on the Synergistic Antioxidant Effect of Tieguanyin and Schizonepeta Extracts[J].China Tea,2021(1):50-53.
Authors:PANG Haiyue  HUANG Lisen  CHEN Yupei  WU Hongtan  CHEN Fangfang  WANG Guihong
Institution:(Department of Public Health and Medical Technology,Xiamen Medical College,Xiamen 361023,China;Engineering Research Center of Natural Cosmeceuticals College of Fujian Province,Xiamen 361023,China)
Abstract:In order to study the synergistic effect of Tieguanyin and Schizonepeta extracts on antioxidation,water extraction method was used to extract the active components.The contents of flavonoids in the two water extracts were determined by rutin method,and the contents of total phenols in the water extracts were 15.13 mg/g(Tieguanyin)and 0.87 mg/g(Schizonepeta Tenuifolia)respectively.The contents of total phenols in the water extracts were determined by gallic acid assay,and the water extracts were 50.87μg/mg(Tieguanyin)and 47.40μg/mg(Schizonepeta Tenuifolia).The DPPH and ABTS methods were applied to assess the antioxidant activities of the two extracts.The results show that the combined use of Tieguanyin and Schizonepeta Tenuifolia extracts had synthetically enhanced the antioxidant effect.
Keywords:synergistic effect  antioxidant  DPPH  ABTS
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