组织培养技术筛选香石竹低玻璃化无性系初报 |
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作者姓名: | 黄宇翔 吴祖建 柯 昉 刘金燕 |
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作者单位: | 福建省农业科学院花卉研究中心 福州350003(黄宇翔,柯昉,刘金燕),福建省农林大学植物病毒研究所 福州350000(吴祖建) |
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基金项目: | 福建省林业厅科技攻关项目“香石竹优质种苗繁育技术研究”(闽林〈2004〉科函9号). |
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摘 要: | 采用离体培养的方法,对香石竹红色品种‘多明格’的茎尖培养试管苗进行连续多代剔除玻璃苗和畸形苗的筛选,继代培养27代后获得了玻璃化率稳定在8%左右的低玻璃化无性系。该无性系经10代的增殖培养,定植到大田后仍然保持母本的优良性状,无明显的有害变异。
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关 键 词: | 香石竹 组织培养 玻璃化 无性系 |
In Vitro Selection for Carnation Clone Against Vitrification |
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Authors: | Huang Yuxiang Wu Zujian Ke fang Liu Jinyan |
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Institution: | 1.Flower Center of Fujian Academy of Agricultural Sciences, Fuzhou 350003; 2. Fuiian Agricultural and Forestry University, Fuzhou 350000 |
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Abstract: | Plantlets in vitro of ''Domingo'', one of the red-flowering carnation breeds, were subcultured in vitro. They were selected for obtaining a clone, whose vitrification rate could be controlled less than 10%, by way of progressive subculture. After 27 times of subculture, such a clone was obtained. After 10 times of multiplying culture, the clone could keep the traits of its original one, and no harmful variation was observed, when they grew in a field. |
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Keywords: | Carnation In vitro selection Clone Vitrification |
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