海产品中牛磺酸测定前处理方法探讨

探讨了海产品中牛磺酸测定的三种前处理方法:6%磺基水杨酸热水浴提取法;0.02mol/L盐酸提取法;70%乙醇沸水浴提取法。结果表明:采用6%磺基水杨酸热水浴提取法均比0.02mol/L盐酸提取法和70%乙醇沸水浴提取法测定值高,测定结果精确度高、重现性好、回收率达98%、变异系数(CV)为1.86%。该法试剂成本低,操作简便,且能同时测定其它18种游离氨基酸,是海产品牛黄酸测定的理想方法。

Study on Pretreatment Method for Determination of Taurine in Marine Products

Three pretreatment methods for the determination of Tautine in marine products were compared ,which were the extraction with 6% sulfosalicyclic acid(SSA) in hot water bath, the extraction with 0.02mol/L hydrochloric acid immerse(HAI) and the extraction with 70% ethyl alcohol circumfluence(EAC)(hot-water bath). The results showed that SSA gave the most accurate determined values, the highest repeatability, recovery rate up to 98.17% and CV 1.86%. The SSA was low in reagent cost and easy to operate. Eighteen kinds of free amino acid could be determined at the same time with SSA, Which is the suitable way for the determination of Taurine analysis of marine products.