| 番茄灰霉病拮抗细菌的筛选 |
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| 引用本文： | 齐爱勇,魏东盛,刘大群,张 汀. 番茄灰霉病拮抗细菌的筛选[J]. 勤云标准版测试, 2006, 0(6) |
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| 作者姓名： | 齐爱勇 魏东盛 刘大群 张 汀 |
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| 作者单位： | 河北农业大学植物保护学院河北省农作物病虫害生物防治工程技术研究中心 保定071001(齐爱勇，刘大群，张汀)，南开大学生命科学学院 天津300071(魏东盛) |
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| 摘 要： | 采集番茄土壤、根、叶片和花样本共96份,经平板法和叶盘法两步分离,获得对番茄灰霉病菌(BotrytiscinereaPers.)有抑制作用的拮抗细菌12株。根据抑菌圈法测定,拮抗细菌对灰霉菌的拮抗能力有显著差异,其中B21菌株抑菌圈直径达35mm,拮抗性最强最稳定。温室试验表明:B21悬浮液和10倍稀释液对番茄灰霉病有较好的防治效果,室内叶片的防效分别为98.68%和98.03%,无显著差异,明显优于45%多菌灵(500倍稀释液)。
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| 关 键 词： | 番茄 灰霉病菌 拮抗细菌 生防菌 |
Screening of Antagonistic Bacteria against Botrytis cinerea |
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| Qi Aiyong,Wei Dongsheng,Liu Daqun,Zhang Ting. Screening of Antagonistic Bacteria against Botrytis cinerea[J]. , 2006, 0(6) |
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| Authors: | Qi Aiyong Wei Dongsheng Liu Daqun Zhang Ting |
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| Affiliation: | 1.Academy of Plant Protection of Hebei Agricultural University, Biocontrol Center of Plant Diseases and Pests of Hebei Province, Baoding 071001; 2.College of Science of Nankai University, Tianjin 300071 |
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| Abstract: | Through plate-method and leaf-disc method, 12 strains of antagonistic bacteria to Botrytis cinerea Pers. were obtained by isolating 96 samples of tomato soils, roots, leaves and flowers. The inhibition zone test showed that the antagonistic bacteria against the pathogen varied with the isolates. Among them, the inhibition zone diameter of isolate B21 was 35 mm, had the strongest and stable inhibiting activity. Indoors, the control effects of B21 suspension and 10 fold diluted culture filtrate on the diseased tomato leaves were 98.68% and 98.03%, which was obviously better than those by 45% carbendazim(500 times). |
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| Keywords: | Tomato Botrytis cinerea Antagonistic bacteria Biocontrol microbe |
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