微卫星开发技术研究进展

对国内外主要应用的6种微卫星开发技术直接文库筛选法、基于锚定PCR技术法、单引物延伸富集法、选择杂交富集法、生物信息学法、转移扩增法进行了综合评价。在结合自己的研究经历及近几年相关的文献报道的基础上，发现目前选择杂交富集法从技术难度、开发效率、开发微卫星的多态性水平3方面综合评价为最有效的微卫星开发技术；传统的直接文库筛选法由于工作量巨大目前报道较少；随着生物数据库核酸序列的不断增加，生物信息学法将会发展成为一种经济、快速、高效的微卫星开发技术；而锚定PCR技术则适合于微卫星文库的筛选，该法可以有效淘汰侧翼序列短而不能设计引物的无效微卫星克隆，因此提高了微卫星开发效率。

Research Development of Microsatellite Exploitation Technologies

In this paper,6 microsatellite exploitation technologies including direct library filter method,anchored-PCR based methods,single primer extension enrichment method,selective hybridization enrichment method,bioinformatics method and transferability amplification method mainly used both at home and abroad were summarized.Based on author's own research experience and the related references reported in recent years,selective hybridization enrichment technology was found to be the most effectual microsatellite ...