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| 山东省局部地区番茄黄化曲叶病毒的分子鉴定 | |
| 引用本文： | 褚栋,侯丽霞,刘国霞,高长生,Judith K Brown. 山东省局部地区番茄黄化曲叶病毒的分子鉴定[J]. 山东农业科学, 2010, 0(2): 13-15,19 |
| 作者姓名： | 褚栋 侯丽霞 刘国霞 高长生 Judith K Brown |
| 作者单位： | 1. 山东省农业科学院高新技术研究中心/山东省作物与畜禽品种改良生物技术重点实验室,山东,济南,250100;农业部黄淮海作物遗传改良与生物技术重点开放实验室,山东,济南,250100 2. 山东省农业科学院蔬菜研究所,山东,济南,250100 3. Department of Plant Sciences,The University of Arizona,Tucson 85721,USA |
| 基金项目： | 国家科技支撑计划重大项目(2006BAD08A18);;农业部公益性行业科研专项项目(200803005) |
| 摘 要： | 近年来山东省部分地区发现番茄黄化曲叶症状。为了确定这种疑似病毒，利用双生病毒外壳蛋白（CP）基因及DNA—A基因组的引物对枣庄、菏泽和淄博的染病番茄叶片进行了检测，测序分析表明这种病毒是番茄黄化曲叶病毒（TYLCV）。系统发育分析结果表明，该病毒可能是由我国东南沿海地区和日本传人的。 |
| 关 键 词： | 番茄黄化曲叶病毒 山东省 PCR |
Molecular Identification of A New Tomato Yellow Leaf Curl Virus in Partial Regions of Shandong Province |
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| CHU Dong,HOU Li-xia,LIU Guo-xia,GAO Chang-sheng,Judith K Brown. Molecular Identification of A New Tomato Yellow Leaf Curl Virus in Partial Regions of Shandong Province[J]. Shandong Agricultural Sciences, 2010, 0(2): 13-15,19 | |
| Authors: | CHU Dong HOU Li-xia LIU Guo-xia GAO Chang-sheng Judith K Brown |
| Affiliation: | CHU Dong,HOU Li-xia ,LIU Guo-xia ,GAO Chang- sheng ,Judith K Brown ( 1. High - tech Research Center,Shandong Academy of Agricultural Sciences/Key Laboratory for Genetic Improvement of Crop,Animal and Poultry of Shandong Province ,Jinan 250100,China; 2. Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai ,Ministry of Agriculture ,Jinan 250100, China; 3. Institute of Vegetables ,Shandong Academy of Agricultural Sciences ,Jinan 250100 ,China; 4. Department of Plant Sciences, The University of Arizona, Tucson 85721, USA) |
| Abstract: | Yellow leaf curl symptoms have been observed in tomato for the first time in many prefectures of Shandong Province during the past several years.To identify the suspect begomovirus,the primers of the coat protein gene and the full length DNA-A genome were used to detect the virus isolated from infected leaves in Zaozhuang,Heze and Zibo.The analysis based on the sequences showed that the virus was tomato yellow leaf curl virus(TYLCV).It might be introduced from southeast coastal area of China and Japan based... |
| Keywords: | Tomato yellow leaf curl virus Shandong Province PCR |
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