基于磁性固定化酶的α-亚麻酸植物甾醇酯合成工艺研究

采用固载有Candida rugosa的磁性固定化酶为生物催化剂，并将其用于α-亚麻酸植物甾醇酯的酶促合成。通过响应面分析优化实验条件，得到最佳工艺参数为：固定化酶用量为25.2mg/mL，甾醇底物浓度为146.5μmol/mL，异辛烷为溶剂，55℃反应5h，得到的植物甾醇的酯化率为92.61%。该固定化脂肪酶经过简单的溶剂清洗可以重复使用10次以上，仍能保持71.8%的催化活性。

Immobilization of Candida rugosa lipase on magnetic polymer microsphere for synthesis of phytosterol esters of alpha-linolienic acid

Immobilized enzyme of Candida rugosa lipase on magnetic microsphere was used as biocatalyst for synthesis of phytosterol esters of alpha-linolienic.Response surface methodology(RSM) was employed to evaluate the effects of synthesis conditions.The optimum synthesis conditions were as follows: 25.2mg/mL immobilized CRL,146.5μmol/mL phytosterols concentration,at 55℃ for 5h in the presence of isooctane,the degree of phytosterols esterification was 92.61%.By solvent cleaning,the immobilization CRL sustained 78.1% activities after having been recycled 10 times.