| 麻疯树cpSSR标记技术的建立与体系优化 |
| |
| 引用本文： | 向倩,周兰英,万静,张旭,雷宝盛,金银春,冯毅,于绪任,赵晓英. 麻疯树cpSSR标记技术的建立与体系优化[J]. 农业科学与技术, 2009, 10(4): 61-64,74 |
| |
| 作者姓名： | 向倩 周兰英 万静 张旭 雷宝盛 金银春 冯毅 于绪任 赵晓英 |
| |
| 作者单位： | 四川农业大学林学院,四川雅安,625014 |
| |
| 基金项目： | National Scientific and Technical Supporting Project of Studies on Superior Species Selecting and Breeding Technique of Jatropha curcas Linn (2007BAD50B01).国家科技支撑课题"麻疯树高油、高产、多抗性良种选育技术研究" |
| |
| 摘 要： | 1材料与方法1．1材料采自我国西南10个地区的麻疯树野生居群的枝条扦插（表1），摘取幼叶用于提取总DNA。
|
| 关 键 词： | SSR标记技术 麻疯树 优化 cp 枝条扦插 野生居群 总DNA 材料 |
Study on Establishment of cpSSR Marker Technology and Optimization of Its Reaction System in Jatropha curcas Linn |
| |
| XIANG Qian,ZHOU Lan-ying,WAN Jing,ZHANG Xu,LEI Bao-sheng,JIN Yin-chun,FENG Yi,YU Xu-ren,ZHAO Xiao-ying. Study on Establishment of cpSSR Marker Technology and Optimization of Its Reaction System in Jatropha curcas Linn[J]. Agricultural Science ＆ Technology, 2009, 10(4): 61-64,74 |
| |
| Authors: | XIANG Qian ZHOU Lan-ying WAN Jing ZHANG Xu LEI Bao-sheng JIN Yin-chun FENG Yi YU Xu-ren ZHAO Xiao-ying |
| |
| Affiliation: | (Forestry College of Sichuan Agricultural University, Ya＇an 625014) |
| |
| Abstract: | [ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA polymerase, Mg^2＋ , DNA template, dNTP and primer were optimized from several levels. [ Result] The optimum concentration of 20 μl reaction system was 10 × Buffer, 2.00 mmol/L Mg^2＋ , 2 U/μl Taq DNA polymerase, 0.2 mmol/L dNTP, 0.2 μmol/L primer and 35 ng/μl DNA template. [ Conclusion] The optimum annealing temperature for cpSSR-PCR reaction system is 52 ℃, and the cpSSR reaction system is steady and reproducible. |
| |
| Keywords: | Jatropha curcas Linn cpSSR-PCR Optimization of reaction system |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
