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| 中华猕猴桃叶片再生体系的建立 | |
| 引用本文： | 尚霄丽,马春华,冯建灿,邢东方,张建鹏. 中华猕猴桃叶片再生体系的建立[J]. 江西农业学报, 2010, 22(4): 50-52 |
| 作者姓名： | 尚霄丽 马春华 冯建灿 邢东方 张建鹏 |
| 作者单位： | 1. 河南农业大学,河南,郑州,450002;濮阳职业技术学院,河南,濮阳,457000 2. 河南农业大学,河南,郑州,450002 3. 河南心连心化肥有限公司,河南,新乡,453731 |
| 摘 要： | 以中华猕猴桃伏牛95-2的组培苗叶片为外植体,研究了不同生长调节物质对不定芽的分化、增殖及生根的影响。结果表明:在MS+ZT1.0 mg/L+NAA0.3 mg/L培养基中,愈伤组织不定芽分化率最高,为87.5%;在MS+6-BA2.0 mg/L+NAA0.5 mg/L培养基中,不定芽增殖系数最高,为4.2;在1/2MS+NAA2.0 mg/L培养基中,不定芽生根率为100%。 |
| 关 键 词： | 中华猕猴桃 组织培养 植株再生 叶片 |
Establishment of Regeneration System from Leaves of Actinidia chinensis |
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| SHANG Xiao-li,MA Chun-hua,FENG Jian-can,XING Dong-fang,ZHANG Jian-peng. Establishment of Regeneration System from Leaves of Actinidia chinensis[J]. Acta Agriculturae Jiangxi, 2010, 22(4): 50-52 | |
| Authors: | SHANG Xiao-li MA Chun-hua FENG Jian-can XING Dong-fang ZHANG Jian-peng |
| Affiliation: | 1.Henan Agricultural University/a>;Zhengzhou 450002/a>;China/a>;2.Puyang Vocational and Technical College/a>;Puyang 457000/a>;3.Henan Xinlianxin Chemical Fertilizer Limited Company/a>;Xinxiang 453731/a>;China |
| Abstract: | Using the leaves of test-tube seedlings of Actinidia chinensis Funiu 95-2 as the explants,the effects of different growth regulating substances on the regeneration,proliferation and rooting of adventitious buds were researched.The results indicated that the highest callus inducement frequency(87.5%) was obtained on MS medium supplemented with 1.0 mg/L ZT and 0.3 mg/L NAA;the best medium for the proliferation of the adventitious buds was MS medium supplemented with 2.0 mg/L 6-BA plus 0.5 mg/L NAA,the prolife... |
| Keywords: | Actinidia chinensis Tissue culture Plant regeneration Leaf |
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