Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia |
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| Authors: | Wenjing Zhang Weihua Jin Delin Sun Luyu Zhao Jing Wang Delin Duan Quanbin Zhang |
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| Institution: | 1Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; E-Mails: (W.Z.); (W.J.); (J.W.); (D.D.);2College of Earth Science, University of Chinese Academy of Sciences, Beijing 100049, China;3Heze Juxinyuan Food Co. Ltd., Heze 274400, China; E-Mails: (D.S.); (L.Z.);4Nantong Branch, Institute of Oceanology, Chinese Academy of Sciences, Nantong 226006, China |
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| Abstract: | Two polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water, respectively. Composition analysis showed that these polysaccharides predominantly consisted of fucose, with galactose, mannose and glucuronic acid as minor components. After degradation and partial desulfation, electrospray ionization mass spectrometry (ESI-MS) was performed, which showed that the polysaccharides consisted of sulfated fucooligosaccharides, sulfated galactofucooligosaccharides and methyl glycosides of mono-sulfated/multi-sulfated fucooligosaccharides. The structures of the oligomeric fragments were further characterized by electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS2 and ESI-CID-MS3). Moreover, the activity of KCA and KCW against the hemolytic activity of both the classical and alternative complement pathways was determined. The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity. In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity. Moreover, the desulfated fractions (ds-DKCA and ds-DKCW) showed less or no activity, which confirmed that sulfate was important for activity. In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway. |
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| Keywords: | polysaccharide Kjellmaniella crassifolia anti-complement |
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