| 酯交换植物油中甾醇测定方法研究 |
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| 引用本文： | 窦巍巍,胡立志,朱秀清,陈昊,于殿宇.酯交换植物油中甾醇测定方法研究[J].东北农业大学学报,2011,42(8):43-47. |
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| 作者姓名： | 窦巍巍 胡立志 朱秀清 陈昊 于殿宇 |
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| 作者单位： | 1. 东北农业大学食品学院,哈尔滨,150030
2. 国家大豆工程技术研究中心,哈尔滨,150050 |
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| 摘 要： | 采用国标铁矾显色法(GB/T 5009.128-2003)测定酯交换植物油中甾醇的含量,通过与改进的Lieberman-Burchard比色法和毛地黄皂苷测定结果进行对比分析,确定了最佳显色剂用量、显色剂组成比例及显色时间.研究结果表明国标铁矾显色法最佳显色条件为:显色剂用量2 mL,显色剂组成比例1:9,显色时间15...
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| 关 键 词： | 植物油 甾醇 酯交换 测定方法 分光光度法 |
Study on determination of sterol in transesterificational vegetable oil |
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| DOU Weiwei,HU Lizhi,ZHU Xiuqing,CHEN Hao,YU Dianyu.Study on determination of sterol in transesterificational vegetable oil[J].Journal of Northeast Agricultural University,2011,42(8):43-47. |
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| Authors: | DOU Weiwei HU Lizhi ZHU Xiuqing CHEN Hao YU Dianyu |
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| Institution: | 1(1.Food College,Northeast Agricultural University,Harbin 150030,China;2.The National Research Centre of Soybean Engineering and Technology,Harbin 150050,China) |
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| Abstract: | The national standard Iron-Alum method(GB/T 5009.128-2003) was studied for etermination of sterol content in the transesterificational vegetable oil,and the optimum coloring conditions were confirmed through analyzed the results and compared with the improved Lieberman-Burchard colorimetric method and digitonin method.The results showed that the optimum coloring conditions of the national standard Iron-Alum method were as following: coloring reagent volume was 2 mL,coloring reagent ratio was 1∶9,and coloring time was 15 min.Therefore,the average relative error and reproducibility is 4.54% and 98.61% by using the national standard Iron-Alum method for determination of high content of sterol in the transesterificational vegetable oil.The method can be used as a quick and simple supervise method for the study of functional oil appended with sterol and industrial product. |
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| Keywords: | vegetable oil sterol transesterification determination method spectrophotography |
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