非洲紫罗兰组培技术研究

研究以非洲紫罗兰幼嫩叶片为外殖体,MS为基本培养基,添加不同浓度6-BA、NAA、GA等外源激素对其愈伤形成、不定芽分化、增殖与不定根形成的影响,试验表明:采用MS+6-BA0.1～2.0mg·L-1+NAA0.01～0.5mg·L-1培养基,愈伤诱导率在20%以上;采用MS+6-BA0.1～1.0mg·L-1+NAA0.01～0.5mg·L-1+GA0.1～1.0mg·L-1培养基,对芽的生长及增殖效果好,并指出GA具有提高不定芽长势和成苗率的作用;采用1/2MS+NAA0.1～0.5mg·L-1或IBA0.1mg·L-1或IAA0.1～0.5mg·L-1,20d内生根率可达97%以上。在腐叶土∶珍珠岩=5∶3的基质中驯苗,成活率达90%以上。

Tissue Culture of Africa Violet

It was studied that the effect of ectohormone on the formation of callus and adventitious root,the differentiation of adventitious bud and the reproduction in the MS media containing 6-BA,NAA and GA were Studied with the young leaf as explant.The result showed that the callus abduction rate of per litre MS medium added 0.1-2.0mg 6-BA and 0.01-0.5mg NAA was over 20%;per litre MS medium added 0.1-1.0mg 6-BA,0.01-0.5mg NAA and 0.1-1.0mg GA was propitious to the bud growth and multiplicatio;GA could improve adventitious bud and seedling;the rooting of 1/2MS medium with NAA 0.1-0.5mg/L,IBA 0.1mg/L or IAA 0.1-0.5mg/L was over 97% within 20d;the surviving of seedling was over 90% in the culture medium,5 soil with rotten leaf and 3 perlite.