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Application of DNA fingerprints for identification and genetic analysis of Carica papaya and other Carica species
Authors:Dror Sharon  Jossi Hillel  Alexander Vainstein  Uri Lavi
Affiliation:(1) Department of Fruit Trees Genetics and Breeding, ARO, P.O. Box 6, 50250 Bet-Dagan, Israel;(2) Department of Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, 76100 Rehovot, Israel;(3) Department of Horticulture, Faculty of Agriculture, The Hebrew University of Jerusalem, 76100 Rehovot, Israel;(4) the Otto Warburg Center for Biotechnology, Faculty of Agriculture, The Hebrew University of Jerusalem, 76100 Rehovot, Israel
Abstract:
Summary Various DNA fingerprint probes were applied to Carica papaya and other Carica species for both identification and genetic analysis.Each of the Carica papaya cultivars is characterized by a specific DNA fingerprints pattern. Various Carica species also have specific patterns which distinguish them from one another. Band sharing levels were used to estimate the relatedness between the various Carica species.Genetic analysis of 11 progeny from a cross between the Carica papaya cultivars 17/82 and 112 suggests that application of DNA fingerprinting to Carica papaya breeding, could make the process more efficient. Genetic analysis of the DNA fingerprint bands revealed no linkage or allelic relationship among the bands analyzed, indicating that these loci are not clustered in the Carica genome.
Keywords:breeding  Carica papaya  DNA markers  genetics
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