鸡Hsc70基因5′侧翼区SNP与耐热性状的相关分析

为研究Hsc70基因作为鸡抗病育种中一种分子标记的可能性,本试验利用克隆测序技术对清远麻鸡、广东温氏矮脚黄鸡、灵山鸡和隐性白羽洛克鸡4个品种共20个个体Hsc70基因的5′侧翼区进行多态性筛查,发现10处变异,包括8个SNPs和2个插入/缺失突变。通过分析,选取C.-521A〉C位点,利用PCR-RFLP方法分析了此位点在灵山鸡和隐性白羽洛克鸡群体中的基因型分布,所得分型结果与测定的耐热性状（T3、皮质酮、CD3＋、CD4＋和CD8＋）进行关联分析,结果表明：常温状态下（15℃）,在隐性白羽洛克鸡群体中,位点C.-521A〉C与CD3＋、CD4＋和CD8＋显著相关（P〈0.05）,其中AA基因型个体CD3＋值显著低于AC基因型个体,而CD4＋与CD8＋值显著高于AC基因型个体;热应激状态下（35℃）,在灵山鸡群体中,位点C.-521A〉C与CD8＋极显著相关（P〈0.01）,CC基因型个体CD8＋值极显著高于AA和AC基因型。

Association analysis of 5rflanking region of Hsc70 gene polymorphism and thermo tolerance traits in chicken

The Hsc70 cytosolic chaperone system facilitates competing pathways of protein folding and degradation. In order to value the effect that the HscTO gene acts as a genetic marker in chicken disease resistance,single nucleotide polymorph isms（SNPs） in the 5＇flanking region of the Hsc70 gene were screened by clone sequencing in 20 individuals from 4 breeds, 10 variations were found,which included 8 SNPs and 2 indel mutations. C.-521A〉C site was genotyped by PCR-RFLP method in Lingshan chicken and white recessive rock. Furthermore, the association of this site with chicken thermo tolerance traits was studied, the result showed that under normal condition（15℃） ,C. -521A〉 C site significantly associated with CD3＋ ,CD4＋ and CD8＋ （P〈0.05） in the white recessive rock,of which value of CD3＋ of AA individual was significantly lower than AC individual,but significantly higher in CD4＋ and CD8＋ value （P〈0.05） ;and under heat stress（35℃） ,C. -521A）C site was significantly associated with CD8＋ （P〈0.01） in the Lingshan chickens,and the CD8＋ value of CC individual was significant higher than the other two genotype individuals.