高能量低蛋白质日粮中添加生物素对蛋鸡脂类代谢的影响

选用300日龄健康海蓝褐蛋鸡90羽,随机分为对照组、病理组、防治组3组（每组3个重复,每个重复10只）,分别饲喂正常日粮（代谢能11.30MJ/kg,粗蛋白质17.00%）、高能量低蛋白质日粮（代谢能12.81MJ/kg,粗蛋白质12.90%）和添加0.3mg/kg生物素的高能量低蛋白质日粮,试验期60d,分别于试验的1,30,60d,每组选取体质量相近的试验蛋鸡6羽,采血制备血清和采集肝脏样品及腹脂,测定血脂含量、肝脂率和腹脂率,观察其病理学变化。结果表明,与对照组相比,试验30,60d,高能量低蛋白质日粮组鸡产蛋性能、HDL-C含量显著降低,TG、TC、LDL-C、ALT、AST、肝脂率和腹脂率显著升高,肝脂肪变性明显。试验30d,与对照组比较,防治组鸡产蛋性能、HDL-C、TG、TC、LDL-C、ALT、AST、肝脂率和腹脂率差异不显著,肝脏脂肪变性不明显;与高能量低蛋白质日粮组比较,防治组鸡产蛋性能、HDL-C含量显著升高,TG、TC、LDL-C、ALT、AST、肝脂率和腹脂率显著降低。60d时,与对照组相比,防治组鸡产蛋性能、HDL-C显著降低,TG、TC、LDL-C、ALT、AST、肝脂率和腹脂率显著升高,肝脏呈现一定的脂肪变性;与高能量低蛋白质日粮组比较,防治组鸡产蛋性能、HDL-C含量显著升高,TG、TC、LDL-C、ALT、AST、肝脂率和腹脂率显著降低。结果提示,采用高能低蛋白日粮饲喂蛋鸡可以成功的建立蛋鸡脂肪肝出血征病理模型;在高能量低蛋白质日粮中添加0.3mg/kg生物素后,可显著影响蛋鸡的脂肪肝出血征蛋鸡脂类代谢,一定程度上对其具有预防作用。

Effect of high-energy low-protein diet supplemented with biotin on fat metabolism of laying hens

The experiment was conducted to investigate the influence of biotin on fat metabolism and fatty liver hemorrhagic syndrome（FLHS） in laying hens.One hundred and thirty five healthy birds（Hy-line Variety Brown laying hens,Gallus domesticus） aged 300 days were randomly allotted into 3 dietary treatments of 45 broilers each.The groups were：1） group 1（control,semisynthetic commercial layer standard diet in accordance with the nutrient requirements of poultry（National Research Councile,1998））;2） group 2（high energy-low protein diet which were partially formulated in accordance with controls;3） group 3（the high energy-low protein diet supplemented with 0.3 mg biotin/kg DM）.All birds were reared for 60 days.Nine hens from each group were selected to collecte samples including serum and liver on 1,30,60 day,respectively.The serum indexs relevant to fat metabolism in serum and/or liver were investigated.The results as follows：as compared to the control,laying performance and HDL-C on the 30th and 60th day in the group 2 decreased.TG,TC,LDL-C,ALT,AST in serum and the ratio of liver fat and abdominal fat increased.Laying performance,HDL-C,TG,TC,LDL-C,ALT,AST in serum and the ratio of liver fat and abdominal fat in the group 3 on the 30th day have no difference.Laying performance and HDL-C on the 60th day in the group 3 decreased.TG,TC,LDL-C,ALT,AST in serum and the ratio of liver fat and abdominal fat increased.As compared to group 2,laying performance and HDL-C on the 30th and 60th day in the group 3 decreased.TG,TC,LDLC,ALT,AST in serum and the ratio of liver fat and abdominal fat increased.The results suggested that high energy-low protein diet can be used for the pathology model building of FLHS.The high energy-low protein diet supplemented with 0.3 mg biotin/kg DM may affect fat metabolism in laying hen and prevent FLHS.