子宫内膜炎患牛子宫内膜PGR和ESR-1 mRNA的表达特征

为探讨卵巢激素及其子宫内膜受体在奶牛子宫内膜炎中的作用,以产后6～10d健康及患急性化脓性子宫内膜炎的中国荷斯坦奶牛为研究对象,通过ELISA法检测子宫内膜中PGF2a,血清中孕酮和雌二醇浓度,SYBRGreenⅠ荧光定量PCR检测子宫内膜PGR和ESR-1mRNA的表达。结果显示,病牛子宫内膜PGF2α水平为（6.503±0.122）μg/L,血清孕酮为（0.816±0.281）μg/L,血清雌二醇为（35.462±2.523）μg/L,而对照组分别为（14.408±0.285）,（0.543±0.142）,（37.6±1.317）μg/L;子宫内膜炎患牛组PGF2a水平极显著低于对照组（P〈0.01）,孕酮含量显著高于对照组（P〈0.05）,PGF2a与孕酮水平呈显著负相关（r=0.893）,雌二醇含量组间差异不显著。PGR基因的mRNA水平极显著高于对照组（P〈0.01）,而ESR-1基因的mRNA水平低于对照组,且差异极显著（P〈0.01）。上述结果表明,孕酮浓度显著升高及PGR mRNA高表达,可能抑制子宫收缩;ESR-1mRNA低表达使子宫新陈代谢减慢,子宫内膜合成PGF2a功能下降,这些改变有可能促进了产后子宫内膜炎的发生发展。

PGR and ESR-1 expression profiles in bovine endometrium with endometritis

To analyze the role of ovarian hormone and its receptors in endometrium with bovine endometritis, we se- lected healthy Chinese Holstein cows and those suffering from acute purulent endometritis at 6-10 days post-natal as control and experimental group, respectively. The concentration of PGF2a in endometrium, progesterone, estradiol in blood serum was detected by ELISA. PGR and ESR-1 expression were examined using real-time PCR in the bovine endometrium. The concentration of PGF2a in endometrium, progesterone, estradiol in blood serum was （6. 503 ± 0. 122）,（0. 816±0. 281）,（35. 462± 2. 523） μg/L in experimental group,and （14. 408±0. 285）, （0. 543±0. 142）, （37.6± 1. 317） μg/L in control group,respectively. The concentration of PGF2a was lower （P〈0.01） ,progesterone in the experimental group was higher than in control group（P 〈0.05）, PGF2a was negatively correlated with pro- gesterone（r=0. 893）. Estradiol was no notable different between control and experimental group（P〉0.05）. The ex- pression level of PGR mRNA was higher notably in experimental group than that in control group（P〈0.01）, but ESR-1 mRNA was lower with significant differenee（P〈0.01）. High progesterone and PGR mRNA levels could in- hibit the contraction of the muscles in the wall of the uterus. Lower estrogen and ESR-I mRNA could down-regulate metabolism of uterus,the functions of uterus synthesise PGF2a. Abnormal expression of PGR and ESR-1 may pro- mote the development of postpartum endometritis.