大曲中细菌和酵母菌的分离及其Biolog微生物系统分析鉴定

[目的]初步探讨Biolog微生物自动分析系统在大曲细菌、酵母菌鉴定中的应用。[方法]利用稀释平板法,采用营养琼脂培养基和麦芽汁培养基,分别对单粮型大曲样品中的细菌和酵母菌进行分离培养。对分离纯化的细菌和酵母菌,经菌落观察和镜检后,利用Biolog微生物自动分析系统对其进行鉴定。[结果]从大曲单个菌落样品中分别分离出7株纯化的细菌菌株和4株纯化的酵母菌株,经Biolog微生物自动分析系统,确定其中5株细菌分别为:M1为短小芽孢杆菌(Bacillus pumilus)、M2为青岛芽孢杆菌(Bacillus qingdaonen-sis)、M3为巨大芽孢杆菌(Bacillus megaterium)、M4为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)、M5为巨大芽孢杆菌(Bacillus megateri-um);确定其中2株酵母菌分别为:N1为产香酵母(Zygosaccharomyces cidri);N2为布拉酵母菌(Saccharomyces boulardii)。[结论]该研究为大曲中的微生物分析奠定了基础。

Study on Color-protection Technique of Tricholoma matsutake in Flexible Packages

[Objective] The study aimed to discuss the color-protection effect of 3 color-protection agents on Tricholoma matsutake in flexible packages.[Method] With the vita min C,chlorine dioxide and sodium sulfite as the color-protection agents,the contrast test was conducted on their color-protection effects of T.matsutake in flexible packages.The effects of the adding amount,temperature and time on the color-protection effect of the vita min C through the single factor experiment were studied and the relevant model was established with the response surface method on 3 influencing factors to deter mine the best conditions for the color-protection of the vita min C.[Result] The contrast test on 3 color-protection agents showed that the vita min C had better color-protection effect,while the chlorine dioxide and sodium sulfite wasn't applicable to the color-protection of T.matsutake.The optimized test with the response surface method showed that,the optimal color protection conditions were as follows:the salt-soaked material was added into the clear water with 3 times of the mushroom weight,adding vita min C according to the weighs of 9.2 g per 1 kg mushroom,heating to 91 ℃ and protecting the color for 20 min,and then desalination,which could get an ideal color-protection effect.[Conclusion] Vita min C had an obvious effect on controlling the non-enzymatic browning in the color-protection of T.matsutake,with good color-protecting effect and long effect time.