不同基因型苹果的离体再生特性及其对NaN_3的反应

不同基因型苹果叶片培养物再生特性不同，主要表现在再生频率的高低和再生类型的类异，通过培养基中的激素和培养过程的光照调节，可以有效地提高各基因型品种的再生频率。不同基因型苹果叶片培养物对ＮａＮ３诱变处理的反应不同，总体而言，其存活率和再生频率均随ＮａＮ３浓度的提高而下降。不同基因型苹果的适宜ＮａＮ３浓度应有所不同，以３０％存活率为标准，对Ｍ２６、嗄拉，首红，长富２及岩富进行诱变处理的适宜浓度分别为３

EFFECT OF GENOTYPE OF APPLE ON in vitro REGENERATING CHARACTERISTICS AND ITS in vitro INDUCING MUTATION WITH NaN 3

Different genotypes of apple varieties were found to have different rates and types of regeneration. The rate of regeneration can be efficiently raised by adjustment of level of plant hormones in media and intensity of illumination in culturing period. When treating with different levels of NaN 3, the survival rates of all 5 varieties decreased with rising level of NaN 3. The moderate level of NaN 3 for M 26 , Gala, Red chief, Changfu No.2 and Yanfu No.10 is 3mmol/L,3mmol/L,3mmol/L,2mmol/L and 2mmol/L, respectively.