| 利用28S rDNA D1/D2区和ITS rDNA序列鉴定甜瓜白粉病病原菌 |
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| 引用本文： | 刘建利.利用28S rDNA D1/D2区和ITS rDNA序列鉴定甜瓜白粉病病原菌[J].植物保护学报,2011,38(1):47-51. |
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| 作者姓名： | 刘建利 |
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| 作者单位： | 北方民族大学生物科学与工程学院,宁夏,银川,750021 |
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| 基金项目： | 宁夏回族自治区自然科学基金(NZ0958) 作者简介: 刘建利,男,1973年生,讲师,研究方向为微生物分子生物学, email: ljl7523@126.com, Tel: 13995006446 |
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| 摘 要： | 为了明确宁夏干旱带压砂甜瓜白粉病病原菌,从病原菌分生孢子中提取DNA,PCR扩增ITSrDNA和28S rDNA D1/D2区段,测序后进行BLAST比对.结果表明,病原菌的ITS rDNA和28SrDNA D1/D2序列与菜豆叉丝单囊壳白粉菌Podosphaera phaseoli、凤仙花又丝单囊壳白粉菌P.bal-saminae、菊科叉丝单囊壳白粉菌P.fwca、苍耳单囊壳白粉菌P.xanthii、瓜类单囊壳白粉菌P.fuligi-nea等叉丝单囊壳属Podosphaera的多个种的ITS rDNA和28S rDNA D1/D2序列之间相似度均大于99%,鉴定甜瓜白粉病病原菌为叉丝单囊壳属Podosphaera.
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| 关 键 词： | 甜瓜白粉病 ITS rDNA 28S rDNA D1/D2 |
| 收稿时间： | 2010/4/29 0:00:00 |
Identification of pathogen of melon powdery mildew with 28S rDNA D1/D2 and ITS rDNA |
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| Liu Jianli.Identification of pathogen of melon powdery mildew with 28S rDNA D1/D2 and ITS rDNA[J].Acta Phytophylacica Sinica,2011,38(1):47-51. |
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| Authors: | Liu Jianli |
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| Institution: | College of Biological Sciences and Engineering, Beifang University for Nationalities, Yinchuan 750021, Ningxia Hui Autonomous Region, China |
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| Abstract: | In order to investigate the pathogen of melon powdery mildew in lands overlaid with sands in Ningxia droughty area, ITS rDNA and 28S rDNA D1/D2 of pathogen amplified by PCR were sequenced and analyzed by BLAST in GenBank after DNA had been extracted from conidia of pathogen. The results showed that ITS rDNA and 28S rDNA D1/D2 sequence of pathogen had more than 99% of similarity to the species of Podosphaera phaseoli, P.balsaminae, P.fusca, P.xanthii, P.fuliginea, suggesting the pathogen of melon powdery mildew belonged to Podosphaera. |
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| Keywords: | melon powdery mildew ITS rDNA 28S rDNA D1/D2 |
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