蒲黄醇提取物对C57BL／6荷瘤小鼠免疫功能的影响

[目的]研究蒲黄醇提物对C57BIM6荷瘤小鼠免疫功能影响。[方法]通过建立Lewis肺癌小鼠移植瘤动物模型，以抑瘤率为指标考察蒲黄醇提物的体内抗肿瘤活性，分别测定其抑瘤率、胸腺指数和脾脏指数；四氮唑盐（MTY）法对荷瘤小鼠脾淋巴细胞增殖活性进行测定；ELISA法测定荷瘤小鼠血清中白细胞介素一2（IL-2）、肿瘤坏死因子-a（TNF-a水平。[结果]蒲黄醇提取物100mg／kg·d组对肿瘤生长有明显的抑制作用（P〈0．01），其抑瘤率为48．3％，蒲黄醇提物三个剂量组均能提高荷瘤小鼠的胸腺指数和脾脏指数。同时100和200mg／kg·d组能显著提高荷瘤小鼠脾淋巴细胞的增殖能力和荷瘤小鼠血清中IL-2、TNF—ol的水平。[结论]蒲黄醇提物具有提高荷瘤小鼠体液免疫和细胞免疫的作用。

Effects of Ethanol Extract of POLLEN TYPHAE on Immune Function in C57BL/6 Tumor Bearing Mice

[Objective] To study the effects of ethanol extract of POLLEN TYPHAE(EEPT) on immune function in C57BL/6 tumor bearing mice.[Method] Taking tumor inhibition rate as index,antitumor activity of EEPT in vivo was investigated by establishing transplanted Lewis tumor mice.The tumor inhibition rate,spleen index and thymus index were respectively determined.The proliferation of spleen lymphocyte and contents of IL-2 and TNF-α in tumor bearing mice were respectively determined by MTT assay and ELISA method.[Result] 100 mg/kg.d EEPT had a significant inhibitory action on the growth of tumor,whose tumor inhibition rate was 48.3%.The three dose groups of EEPT could raise spleen index and thymus index.Meanwhile,100 and 200 mg/kg·d EEPT could significantly increase not only proliferation of spleen lymphocyte,but also the content of IL-2 and TNF-α.[Conclusion] EEPT could promote the function of humoral immunity and cellular immunity in C57BL/6 tumor bearing mice.