Immunolocalization of Pepper mild mottle virus in Capsicum annuum seeds |
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| Authors: | Yoshikatsu Genda Kyoko Sato Osamu Nunomura Tetsuo Hirabayashi Jun Ohnishi Shinya Tsuda |
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| Affiliation: | (1) Japan Horticultural Production and Research Institute, Chiba, Japan;(2) Department of Plant Protection, National Institute of Fruit Tree Science, Tsukuba, Japan;(3) Department of Plant Pathology, National Agricultural Research Center, Tsukuba 305-8666, Japan |
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| Abstract: | To clarify the mechanism of seed transmission of Pepper mild mottle virus (PMMoV), the virus was immunolocalized in Capsicum annuum seeds using fluorescence microscopy. Two distinct patterns were observed: In the first, PMMoV was present in the epidermis and parenchyma but not in the endosperm or embryo; in the second, the virus was restricted to the surface of the epidermis and parenchyma. These findings shed light on the fundamental mechanisms of seed transmission of tobamoviruses and may aid in the design of new methods to prevent the spread of seedborne virus diseases. |
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| Keywords: | Capsicum annuum Immunolocalization Pepper mild mottle virus Seed transmission Trisodium phosphate Tobamoviruses |
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