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奶牛冷冻胚胎规模化移植效果研究
引用本文:林峰,陈玉霞,史明艳,高腾云,杨美广.奶牛冷冻胚胎规模化移植效果研究[J].中国农学通报,2006,22(7):9-11.
作者姓名:林峰  陈玉霞  史明艳  高腾云  杨美广
作者单位:1. 河南农业大学牧医工程学院,郑州,450002
2. 西北农林科技大学动物科技学院,陕西,杨凌,712100
3. 河南省濮阳县农牧局,濮阳县,457100
基金项目:河南省科普及传播工程项目(2004209196).
摘    要:为了提高奶牛良种化程度,对奶牛冷冻胚胎的规模化移植效果进行了研究。结果表明:PG二次肌注法的发情率显著高于(P<0.05)PG一次肌注法,两种方法的发情同期率均超过85%,受体牛发情时间多集中在肌注PG后48~72h;胚胎解冻后的复苏率达到82.58%,且早期囊胚与囊胚的复苏率要高于桑椹胚(P>0.05);共移植受体牛218头,受胎97头,受胎率为44.5%,且春、秋季的移植效果差异不显著(P>0.05)。为奶牛冻胚的规模化移植研究提供了参考依据。

关 键 词:奶牛  冷冻胚胎  同期发情  胚胎移植
收稿时间:2005-04-28
修稿时间:2005-04-282005-05-25

Effect of the Scaled Tranfer on Cryopresrved Embryos of Cows
Lin Feng,Chen Yuxia,Shi Mingyan,Gao Tengyun,Yang Meiguang.Effect of the Scaled Tranfer on Cryopresrved Embryos of Cows[J].Chinese Agricultural Science Bulletin,2006,22(7):9-11.
Authors:Lin Feng  Chen Yuxia  Shi Mingyan  Gao Tengyun  Yang Meiguang
Institution:1.College of Animal Husbandry and Veterinary, Henan Agricultural University,Zhengzhou 450002; 2.College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry,Yangling Shaanxi712100; 3.Bureau of Agriculture and Animal Husbandry, Puyang County of Henan Province, Puyang County 457100
Abstract:In order to improve the purebred level of cows, the transfer effect on the cryopreserved embryos of cows was researched.The results showed that the estrous rate of two-injection method of PG was obviously higher than that of one-injection method of PG(P<0.05),and the synchronous estrus rate of the two methods were all higher than 85%,and the estrous time of the recipients was concentrated on 48-72 hours after intramuscular Injection of PG;The revival rate of the embryos was 82.58% after they were thawed , and the revival rate of the early blastocysts and blastocysts were higher than that of the morulae(P>0.05) ; 218 recipients were transferred and 97 recipients were pregnant, and the pregnancy rate were 44.5%. This study provided the reference basis for the large-scaled transfer of the cryopreserved embryos of cows.
Keywords:Cow  Cryopreserved embryo  Synchronous estrus  Embryo transfer
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