红叶石楠快繁条件优化

目的]为了优化红叶石楠的快繁条件。方法]通过L16(43)正交试验,配置不同基础培养基、不同植物激素种类及水平对红叶石楠不定芽进行增殖培养。结果]不同因素影响红叶石楠快繁的大小顺序为:基础培养基>6-BA>NAA。B5培养基为最佳培养基。6-BA浓度为1.0～2.0mg/L时不定芽数变化不明显;6-BA浓度为2.0～4.0mg/L时不定芽数随浓度的提高显著增加,在浓度为4.0mg/L时最多,平均增殖系数达到22.38,继续增加6-BA浓度到8.0mg/L时不定芽数则明显减少。NAA浓度为0.05～0.20mg/L时不定芽数随NAA浓度的提高而增加,在浓度为0.20mg/L时平均增殖系数最高,达到20.25;当NAA浓度从0.20mg/L逐渐增加到0.40mg/L时不定芽数呈下降趋势。因此确定最优水平组合为B5+6-BA4.0mg/L+NAA0.20mg/L。结论]该方法可快速优化红叶石楠的快繁条件,有效提高红叶石楠的增殖效率。

Optimization on Culture Conditions for Rapid Propagation of Photinia fraseri by Orthogonal Design

Objective] The aim was to study the optimal culture conditions of the rapid propagation of Photinia fraseri. Method] The different basic media, the phytohormones and their different levels were chose to culture adventitious bud of Photinia fraseri to optimize the culture conditions by orthogonal experimental design. Result] The effect of different influences on rapid multiplication of Photinia fraseri was in order: Basal medium>6-BA>NAA. And B5 was the best medium. The number of adventitious bud had an light change when the concentration of 6-BA was 1.0-2.0 mg/L. The number of adventitious bud increased obviously when the concentration of 6-BA was 2.0-4.0 mg/L, and that was most with average multiplication coefficient 22.38 at 4.0 mg/L. But the number of adventitious bud decreased obviously when the concentration of 6-BA was 4.0～8.0 mg/L. The number of adventitious bud increased obviously when the concentration of NAA was 0.05-0.20 mg/L. But the number of adventitious bud decreased when the concentration of NAA was 0.20-0.40 mg/L.The best treatment for subculture of Photinia fraseri was B5 + 6-BA 4.0 mg/L + NAA 0.2 mg/L. Conclusion] The culture conditions for rapid propagation of Photinia fraseri could be optimized with the experiments based on the orthogonal design, then effectively improved the efficiency of rapid propagation of Photinia fraseri.