番茄斑萎病毒TSWV 的鉴定及抗病种质的筛选

 在北京顺义地区番茄栽培温室中随机抽取了15份疑似感染番茄斑萎病毒（TSWV）的植株叶片和果实，利用TSWV试剂盒进行DAS-ELISA检测，结果均为阳性，表明其感染了TSWV。利用来自秘鲁番茄的抗TSWV基因Sw-5的共显性SCAR标记，对442份番茄材料进行分析，筛选出24份含有Sw-5的材料，其中4份为野生番茄，1份为杂交种，16份为高代育种自交系，3份为野生潘那利番茄渐渗系。4份野生番茄综合农艺性状较差，如果实小、产量低、品质和风味差等，不能直接应用于育种；其余20份含Sw-5的材料综合农艺性状较好，可作为番茄抗TSWV的育种材料。

Identification of Tomato spotted wilt virus and Screening for Resistant Sources in Tomato

Fifteen tomato plants which seemed infected Tomato spotted wilt virus(TSWV)were selected randomly from the tomato cultivation greenhouse of Shunyi District,Beijing.Then the leaves and fruits were obtained from each plant and tested for the presence of TSWV using a DAS-ELISA kit.The results showed positive,indicating that 15 tomato materials had been infected with TSWV.A genetic analysis of 442 tomato materials with co-dominant SCAR markers developed from Sw-5,TSWV resistance gene originated from Solanum peruvianum,was issued and identified 24 resistant materials,which including 4 wild tomatoes,1 hybrid,16 breeding inbred lines and 3 S.pennellii introgression lines.Those 4 wild tomatoes are not suitable as breeding materials because of the poor agronomic characters,i.e.small fruits,low production,poor quality and bad flavor,while the other 20 tomato materials can be used for developing cultivars resistant to TSWV due to their better agronomic characters.