利用SSR荧光标记构建92个梨品种指纹图谱

 利用SSR荧光标记技术筛选出的10对SSR荧光标记引物，构建中国建国以后选育的92个梨品种的SSR指纹图谱。10对SSR引物共扩增出132个等位基因，位点的杂合度在0.2421 ~ 0.8632之间。10对引物的扩增带型为8 ~ 44个，平均为29.7个。利用其中5对引物KA16、NH009b、NH013a、CH02b121和CH05d04可区分所有供试品种。92个梨品种10个SSR位点的遗传多样性和多态性信息含量的变化范围分别为0.4886 ~ 0.8810和0.4537 ~ 0.8707，平均值分别为0.7920和0.7732。92个梨品种的SSR指纹图谱互不相同，可以作为各品种特定的图谱，为品种鉴别提供依据。

Using the SSR Fluorescent Labeling to Establish SSR Fingerprints for 92 Cultivars in Pyrus

The fluorescent labeling of simple sequence repeat（SSR）detection was used to establish SSR fingerprints of 92 pear cultivars. Ten pairs of SSR primers screened amplified 132 alleles，with an average of 13.2 alleles per locus. The range of locus heterozygosity was 0.2421–0.8632. The amplified bands of 10 pairs of primers were 8–44 with an average of 29.7. KA16，NH009b，NH013a，CH02b121 and CH05d04 can distinguish all of the pear cultivars. The ranges of gene diversity and PIC at 10 SSR loci of 92 cultivars were 0.4886–0.8810 and 0.4537–0.8707 respectively，with an average of 0.7930 and 0.7732 respectively. Ninety-two pear cultivars with different SSR fingerprints of each other can serve as the cultivars-specific patterns and as an important basis for cultivars identification.