‘短柄樱桃’花芽休眠解除过程中差异表达基因的研究

 以中国樱桃的优良品种‘短柄樱桃’（Prunus pseudocerasus Lindl.‘Duanbing’）的花芽为材料，采用mRNA 差异显示技术，分析休眠解除的过程中相关差异表达基因。共克隆获得79 个差异cDNA片段；通过半定量RT-PCR 验证，确定18 个阳性差异cDNA 片段为休眠解除相关候选基因；休眠解除过程中上调的基因片段11 个，下调的7 个。测序及同源性比对结果显示：差异表达基因主要参与糖代谢、信号转导、跨膜转运及氧化还原等反应过程。这些基因在‘短柄樱桃’休眠解除阶段可能起到直接或间接的作用。

Studies on Differentially Expressed Genes in Flower Bud During Dormancy Release in Prunuspseudocerasus Lindl. ＇Duanbing＇

The mRNA differential display technique（DDRT）was used to identify the differentially expressed cDNA fragments during chilling-induced release of dormancy in buds of Prunus pseudocerasus Lindl.‘Duanbing’. Seventy-nine differentially expressed fragments were initially identified by DDRT， and among which，18 of them were confirmed as true differentially expressed genes using semiquantitative RT-PCR. Out of these 18 differentially expressed genes，11 were up-regulated and 7 were down-regulated，respectively，during the release of dormancy. Gene ontology analysis indicated that the differentially expressed genes are mostly involved in the processes of glycometabolism ， signal transduction，trans-membrane transport，and oxidation-reduction reactions. The results suggest that these genes may somehow contribute to the release of dormancy，either directly or indirectly，in Prunus pseudocerasus Lindl.‘Duanbing’.