Prevalence of porcine reproductive and respiratory syndrome virus detection
in aborted fetuses,mummified fetuses and stillborn piglets using quantitative polymerase
chain reaction |
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Authors: | Em-on OLANRATMANEE Piya WONGYANIN Roongroje THANAWONGNUWECH Padet TUMMARUK |
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Abstract: | The objective of the present study was to investigate the prevalence of porcine
reproductive and respiratory syndrome (PRRS) virus detection in aborted fetuses (n=32),
mummified fetuses (n=30) and stillborn piglets (n=27) from 10 swine herds in Thailand
using quantitative polymerase chain reaction (qPCR). Pooled organs and umbilical cord from
each fetus/piglet were homogenized and subjected to RNA extraction and cDNA synthesis. The
qPCR was carried out on the ORF7 of the PRRS viral genome using fluorogenic probes for
amplified product detection. The results revealed that 67.4% (60/89) of the specimens
contained PRRS virus. The virus was found in 65.6% (21/32) of aborted fetuses, 63.3%
(19/30) of mummified fetuses and 74.1% (20/27) of stillborn piglets
(P=0.664). Genotype 1, genotype 2 and mixed genotypes of PRRS virus were
detected in 19.1% (17/89), 25.8% (23/89) and 22.5% (20/89) of the specimens, respectively
(P=0.316). PRRS virus antigen was retrieved from both
non-PRRS-vaccinated herds (68.2%, 45/66) and PRRS-vaccinated herds (65.2%, 15/23)
(P=0.794). These findings indicated that these specimens are important
sources of the PRRS viral load and the viral shedding within the herd. Thus, intensive
care on the routine management of dead fetuses and stillborn piglets in PRRS
virus-positive herds should be emphasized. |
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Keywords: | mummy PRRS qPCR reproduction swine |
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