一株新型鹅星状病毒鉴定及遗传演化分析

近几年，我国主要养鹅地区暴发了一种以关节腔和内脏器官尿酸盐沉积为特征的高度致死的关节型和内脏型痛风，给我国养鹅业造成了巨大的经济损失.多项研究结果表明，引起此次雏鹅痛风的病原是一种新型鹅星状病毒（Goose Astrovirus，GoAstV）.为了解GoAstV在广东地区的流行情况及其遗传变异，本研究对广东地区雏鹅群开展GoAstV流行病学调查，结果表明，GoAstV在广东地区雏鹅群中广泛流行，其阳性率为40%.对GoAstV/Guangdong/DBN-1/2019（GoAstV-DBN）进行全基因组测序和遗传演化分析，结果显示GoAstV-DBN与AHAU2/2018同源性最高.与参考毒株相比广东地区GoAstV未发生明显变异，但GoAstV-DBN Cap蛋白发生了Y36V和L540Q两个氨基酸突变.本研究可为广东地区GoAstV的防控和疫苗毒株的筛选提供理论依据.

Identification and evolutionary analysis of GoAstV in gosling

In recent years, a new type gout with a high lethality has outbreak in gosling in China, which featured with urate deposition in joint and viscera, leading to huge economic loss. Several researches show that, a novel Goose Astrovirus (GoAstV) is the pathogen for this gout outbreak. In order to know about the epidemic and variation of GoAstV in Guangdong province, epidemiology of GoAstV in gosling in Guangdong was performed. The results show that the positive rate of GoAstV in gosling is 40%, which indicates that GoAstV is epidemic in gosling. The evolutionary analysis shows that GoAstV-DBN shares highest homology with AHAU2/2018, and no obvious variation was observed in GoAstV in Guangdong. However, Y36V and L540Q amino acid mutation in Cap protein occurred in GoAstV-DBN. This study provides a basis and reference for the prevention of GoAstV in Guangdong province.