| 梅花鹿角柄骨膜蛋白质组学初步研究 |
| |
| 引用本文： | 徐代勋,王桂武,赵海平,杨颖,宁浩然,李春义.梅花鹿角柄骨膜蛋白质组学初步研究[J].特产研究,2011,33(1):1-4. |
| |
| 作者姓名： | 徐代勋 王桂武 赵海平 杨颖 宁浩然 李春义 |
| |
| 作者单位： | 1. 江苏科技大学,江苏,镇江,212018;中国农业科学院特产研究所,吉林,吉林,132109
2. 中国农业科学院特产研究所,吉林,吉林,132109 |
| |
| 摘 要： | 为了利用蛋白质组学技术探索鹿茸研究模型中的特异性蛋白，本试验探索了一套完整的梅花鹿角柄骨膜蛋白提取及纯化方法。角柄是雄鹿头上着生的永久性骨桩，鹿茸每年由该骨桩上脱落和再生。本试验采集了梅花鹿角柄骨膜，利用冷冻液氮研磨法提取蛋白样品。蛋白样品分别经过丙酮沉淀和TCA-丙酮沉淀处理。结果显示，TCA-丙酮沉淀处理后电泳图谱斑点清晰，分辨率较高，是双向电泳蛋白提取样品的较优处理方法。
|
| 关 键 词： | 梅花鹿 角柄骨膜 双向电泳 TCA-丙酮沉淀 |
Proteomics Study On Pedicle Periosteum of Sika Deer |
| |
| XU Dai-xun,WANG Gui-wu,ZHAO Hai-ping,Yang ying,Ning Hao-ran,LI Chun-yi.Proteomics Study On Pedicle Periosteum of Sika Deer[J].Special Wild Economic Animal and Plant Research,2011,33(1):1-4. |
| |
| Authors: | XU Dai-xun WANG Gui-wu ZHAO Hai-ping Yang ying Ning Hao-ran LI Chun-yi |
| |
| Institution: | (2※) (1.School of Biotechnology and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang 212018, China;2.Institute of Special Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Jilin 132109,China) |
| |
| Abstract: | To develop an optimal proteomics technology for extracting specific proteins from periosteum for deer antler research,we constructed a suite of methods for protein extraction and purification from pedicle periosteum.Fresh pedicle periosteum was collected from 1-year-old male sika deer.Pedicles are the deer cranial permanent bony protuberances, from which antlers cast and regenerate.Protein was extracted from the frozen periosteum in liquid nitrogen.Two different methods,Acetone and TCA-Acetone precipitation... |
| |
| Keywords: | Sika deer Pedicle periosteum Two-dimensional electrophoresis TCA-Acetone precipitation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
