| 反相高效液相色谱法同时测定红松种仁中的三种内源激素 |
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| 引用本文： | 陈正波,张莹,陈小强.反相高效液相色谱法同时测定红松种仁中的三种内源激素[J].特产研究,2011,33(1):36-38. |
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| 作者姓名： | 陈正波 张莹 陈小强 |
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| 作者单位： | 1. 吉林省白山市江源区大阳岔国营林场,吉林,白山,134300
2. 东北林业大学森林植物生态学教育部重点实验室,哈尔滨,150040 |
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| 基金项目： | 中央高校基金科研业务费专项资金项目 |
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| 摘 要： | 建立了红松种仁中赤霉素、吲哚乙酸、脱落酸等3种内源激素的反相高效液相色谱检测方法。采用HiQ-SilC18柱（4．6mm×250mm，5μm），以甲醇-水-冰乙酸（45．0：54．2：0．8，V：V：Ⅵ混合溶液为流动相，流速为1．0mL／min，进样量为10μL，检测波长为254mm，柱温为40℃，用外标法进行定量测定。GA3、IAA和ABA的最低检测限分别为0．5mg／L、0．2mg／L和0．1mg／L，平均回收率分别为95．2％、97．2％和94．1％，相对标准偏差分别为2．3％、1．7％和1．3％。该方法具有简便、准确和易操作等特点。
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| 关 键 词： | 反相高效液相色谱 红松种仁 内源激素 |
Simultaneous Determination of Three Endogenous Hormones in Pinus koraiensis Nuts by Reversed phase High Performance Liquid Chromatography |
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| CHEN Zheng-bo,ZHANG Ying,CHEN Xiao-qing.Simultaneous Determination of Three Endogenous Hormones in Pinus koraiensis Nuts by Reversed phase High Performance Liquid Chromatography[J].Special Wild Economic Animal and Plant Research,2011,33(1):36-38. |
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| Authors: | CHEN Zheng-bo ZHANG Ying CHEN Xiao-qing |
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| Institution: | CHEN Zheng-bo1,ZHANG Ying2,CHEN Xiao-qing(2※) (1.Dayangcha State Forest Farm,Jiangyuan District,Baishan 134300,China;2.Key Lanboratory of Forest Plant Ecology,the Ministry of Educaion,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | A reversed-phase high performance liquid chromatography（RP-HPLC） method was developed for the simultaneous determination of gibberellin acid（GA3）,indole-3-acetic acid（IAA）,and abscisic acid（ABA） in Pinus Koraiensis nuts.The method was performed with a HiQ-SiL C18 column（250 mm×4.6 mm,5μm） using methanol-water -acetic acid（45.0:54.2:0.8,V:V:V） as mobile phase,sample injection at the velocity of 1.0 mL/min with an input of 10μL,and detection wavelength at 254 nm.,column temperatuve 40℃.The detection limit of GA3,IAA and ABA were 0.5, 0.2 and 0.1mg/l,respectively.The average recoveries were 95.2%、97.2%and 94.1%,respectively.The relative standard deviations were 2.3%、1.7%and 1.3%,respectively.The method is convenient,fast and accurate. |
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| Keywords: | Reversed-phase high performance liquid chromatographic(RP-HPLC) Pinus koraiensis nuts Exogenous hormone |
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