| 应用PCR方法检测鸡传染性贫血病毒 |
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| 引用本文： | 杨兵,徐福洲,王金洛,陈小玲,孟彦妮.应用PCR方法检测鸡传染性贫血病毒[J].华北农学报,2003,18(3):90-92. |
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| 作者姓名： | 杨兵 徐福洲 王金洛 陈小玲 孟彦妮 |
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| 作者单位： | 北京市农林科学院畜牧兽医研究所,北京,100089 |
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| 基金项目： | 北京市科委合同项目(954414900) |
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| 摘 要： | 根据鸡传染性贫血病毒(CIAV)基因组序列，设计一对引物扩增基因组上nt358～nt1042之间684bp DNA片段。在CIAV感染MDCC-MSBl细胞系中提取核酸为模板可扩增出相应DNA片段，且以感染细胞核酸为模板反应敏感性可达1fg，而以其他病原核酸为模板扩增结果均为阴性。应用该PCR方法对实验感染鸡肝脏、脾脏、胸腺、肾脏、法氏囊、骨髓、白细胞、泄殖腔棉拭子等不同样品进行检测，均可扩增出相应DNA片段。证明我们建立的PCR方法敏感性特异性强，可用于临床感染不同组织CIAV的检测。
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| 关 键 词： | 应用 PCR方法 检测技术 鸡 传染性贫血病毒 基因组序列 反应敏感性 临床感染 |
| 文章编号： | 1000-7091(2003)03-0090-03 |
| 修稿时间： | 2002年12月27 |
Detecting Chicken Infectious Anemia Virus with Polymerase Chain Reaction |
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| YANG Ring,XU Fu-zhou,WANG Jin-luo,CHEN Xiao-ling,MENG Yan-ni.Detecting Chicken Infectious Anemia Virus with Polymerase Chain Reaction[J].Acta Agriculturae Boreali-Sinica,2003,18(3):90-92. |
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| Authors: | YANG Ring XU Fu-zhou WANG Jin-luo CHEN Xiao-ling MENG Yan-ni |
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| Abstract: | A polymerase chain reaction (PCR) was constructed to detect chicken infectious anemia virus (CIAV). The assay used a pair of primers to amplify a 684 bp fragment of CIAV genome which located in the position 358 to 1 042. The amplified fragment was observed to detect infected cell culture and the sensitivity of the assay can be up to 1 fg. The result of amplification with NDV,MDV,IBDV, IBV,EDSV and E. Coli were negative. It showed that the assay was specific and highly sensitive. The positive fragments sere also be achieved in infected chicken tissues such as liver, spleen, thymus, bursa, kidney, white blood cell and rectal sub. |
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| Keywords: | Chicken Chicken infectious anemia Virus PCR |
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