Age-related changes in leukocytes and T cell subsets in peripheral blood of Japanese Black cattle |
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Authors: | Sachi TANAKA Kohtaro MIYAZAWA Atsuko KUWANO Kouichi WATANABE Shyuichi OHWADA Hisashi ASO Shigeru NISHIDA Takahiro YAMAGUCHI |
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Institution: | Department of Animal Biology, Graduate School of Agricultural Science, Tohoku University, Aoba, Sendai, Japan |
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Abstract: | It is well known that the immune system changes with age during development and maturation in Holstein cattle. But age-related changes in leukocytes and T cell subsets in peripheral blood of Japanese Black cattle still remain unclear. The aim of the present study was to investigate comparative changes of leukocytes (granulocytes, monocytes, B cells and T cells) and T cell subsets (CD4+, CD8+, γδ, CD8+γδ and WC1+γδ T cells) in Japanese Black cattle aged 0.5, 1, 2, 6, 18 and 36–41 (adult) months on flow cytometry using specific monoclonal antibodies for the cell surface markers. T cell proportion was approximately 40% in 2-month-old cattle and decreased to 20.6% in adults. In contrast, B cell proportion significantly increased from 7.4% to 28.2% with age. In T cell subsets the percentage of CD4+ T cells significantly increased from 40.5% to 60%, but that of WC1+γδ T cell subset significantly decreased with age. The percentages of CD8+ and CD8+γδ T cells did not change. The present study details the proportional changes in leukocyte and T cell subsets with age in the peripheral blood of Japanese Black cattle and these findings are similar to those described for Holstein cattle. |
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Keywords: | aging Japanese Black cattle leukocytes T cell subsets |
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