| 发根农杆菌A4菌株诱导三分三毛状根的研究 |
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| 引用本文： | 杨小蕊,张明生,曹然.发根农杆菌A4菌株诱导三分三毛状根的研究[J].贵州大学学报(农业与生物科学版),2011,30(2):115-119. |
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| 作者姓名： | 杨小蕊 张明生 曹然 |
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| 作者单位： | 贵州大学生命科学学院,贵州省中药材繁育与种植工程实验室,贵州贵阳550025 |
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| 基金项目： | 贵州省中药材现代产业技术体系建设项目(GZCYTX2010-0201) |
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| 摘 要： | 利用发根农杆菌A4菌株侵染三分三组培苗叶片诱导毛状根,并对其毛状根进行继代增殖培养;采用PCR技术检测毛状根。结果表明：发根农杆菌A4菌株Ri质粒的rolB基因已整合到三分三叶肉细胞的基因组中,进而成功地从叶片诱导出毛状根,诱导率可达77%以上;毛状根除菌后,能在无激素的MS固体培养基上快速生长。三分三遗传转化体系的建立为实现基于毛状根的三分三产托品烷类生物碱的代谢工程奠定基础。
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| 关 键 词： | 三分三 发根农杆菌A4菌株 遗传转化 毛状根 |
Hairy Root Induction of Anisodus acutangulus by Agrobacterium rhizogenes A4 |
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| YANG Xiao-rui,ZHANG Ming-sheng,GAO Ran.Hairy Root Induction of Anisodus acutangulus by Agrobacterium rhizogenes A4[J].Journal of Mountain Agriculture & Biology,2011,30(2):115-119. |
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| Authors: | YANG Xiao-rui ZHANG Ming-sheng GAO Ran |
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| Institution: | YANG Xiao-rui,ZHANG Ming-sheng,CAO Ran(College of Life Sciences,Guizhou Engineering Laboratory of Propagation and Cultivation on Traditional Chinese Medicinal Materials,Guizhou University,Guiyang Guizhou 550025,China) |
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| Abstract: | Agrobacterium rhizogenes A4 was used to induce hairy roots by infecting the leaves of Anisodus acutangulus,the hairy roots were subcultured and multiplied in the current investigation.The PCR technique was applied to detect hairy roots.The results showed that rolB gene in Ri plasmid of Agrobacterium rhizogenes A4 was easily integrated into the genome of mesophyll cell of Anisodus acutangulus,and the hairy roots were induced successfully from the leaves at the frequency of more than 77%.The hairy roots that ... |
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| Keywords: | Anisodus acutangulus Agrobacterium rhizogenes A4 Genetic transformation Hairy roots |
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