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| 真菌病害拮抗菌的筛选及其对多种植物病原真菌的拮抗活性测定 | |
| 引用本文： | 汪茜,胡春锦,黄思良,柯仿钢,黎起秦. 真菌病害拮抗菌的筛选及其对多种植物病原真菌的拮抗活性测定[J]. 广西农业科学, 2010, 41(7): 675-678 |
| 作者姓名： | 汪茜 胡春锦 黄思良 柯仿钢 黎起秦 |
| 作者单位： | 1. 广西大学农学院,南宁,530005;广西农业科学院微生物研究所,南宁,530007 2. 广西农业科学院微生物研究所,南宁,530007;广西作物遗传改良生物技术重点开放实验室,南宁,530007 3. 南阳师范学院生命科学与技术学院,河南南阳,473061 4. 广西大学农学院,南宁,530005 |
| 基金项目： | 广西农业科学院科技发展基金重点项目 |
| 摘 要： | 针对广西境内常见多发的12种植物真菌病害,对柑橘、香蕉、甘蔗、辣椒、药用植物等作物根际土壤进行拮抗菌筛选试验。结果从不同作物根际土壤中共分离得到386株菌株,其中有拮抗作用的34株,平皿拮抗效果较好的16株;再经复筛,得到一株对柑橘炭疽菌有较好抑制效果的拮抗菌Bs55;对峙培养试验表明,Bs55菌株对12种常见病原真菌具有较强的抗菌活性,抑菌率最高达87.7%。拮抗菌Bs55具有拮抗作用强、能大量繁殖、抑菌谱广等特点,是具有较好利用前景的生物防治材料。 |
| 关 键 词： | Bs55菌株 枯草芽孢杆菌 病原真菌 拮抗活性 |
Screening of antagonistic bacteria against fungal diseases and determination of their antagonistic activity to various plant pathogenic fungi |
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| WANG Qian,HU Chun-jin,HUANG Si-liang,KE Fang-gang,LI Qi-qin. Screening of antagonistic bacteria against fungal diseases and determination of their antagonistic activity to various plant pathogenic fungi[J]. Guangxi Agricultural Sciences, 2010, 41(7): 675-678 | |
| Authors: | WANG Qian HU Chun-jin HUANG Si-liang KE Fang-gang LI Qi-qin |
| Affiliation: | 1 Agricultural College,Guangxi University,Nanning 530005,China;2 Microbiology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;3 Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,China;4 College of Life Science and Technology,Nanyang Normal University,Nanyang,Henan 473061,China) |
| Abstract: | In order to find out the antagonistic bacterial agents against 12 kinds of crop fungal diseases occurring commonly in Guangxi,screening experiments were carried out using bacteria from rhizospheric soil of citrus,banana,sug-arcane,pepper and some medicinal plants.The studies resulted in isolation of 386 strains from rhizospheric soil of different crops.Among these,34 strains showed antagonistic activity and 16 strains showed good antagonistic effects on plate.After secondary screening,antagonistic bacterial Bs55 was screened out with strong inhibitory effect on citrus anthracnose.Con-front culture tests showed that Bs55 strain had strong antifungal activity to 12 kinds of common pathogenic fungis,the high-est inhibiting rate reached 87.7%.It has the characteristics of strong antagonism and large scale propagation,and was ap-proved to be a promising biocontrol agent with a broad-spectrum biocontrol potential. |
| Keywords: | Bs55 strain Bacillus subtilis pathogenic fungi antifungal activity |
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