Biological control of rice blast by the epiphytic bacterium <Emphasis Type="Italic">Erwinia ananas</Emphasis> transformed with a chitinolytic enzyme gene from an antagonistic bacterium, <Emphasis Type="Italic">Serratia marcescens</Emphasis> strain B2 |
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Authors: | Nobutaka Someya Shinichi Numata Masami Nakajima Akira Hasebe Tadaaki Hibi Katsumi Akutsu |
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Institution: | (1) Faculty of Agriculture, Ibaraki University, Ami-machi, Ibaraki, 300-0393, Japan;(2) National Institute for Agro-Environmental Sciences, Tsukuba, Japan;(3) Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan;(4) Present address: RIKEN (Institute of Physical and Chemical Research) Plant Science Center, Saitama, Japan |
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Abstract: | The gene chiA, encoding for the endochitinase ChiA, was cloned from Serratia marcescens strain B2, a tomato epiphytic bacterium, and introduced into the epiphytic bacterium Erwinia ananas NR-1, isolated from rice phylloplane. The gene chiA was introduced under the control of two types of promoter into a broad-host-range plasmid vector. The vector contained various fragments with promoter activity isolated from E. ananas chromosomal DNA. The constructed vectors were designated pchiA-V1pcf9 and pchiA-V1pcf53 for their respective promoters. E. ananas NR-1 transformed with either of these vectors produced and secreted ChiA. The antifungal activity of ChiA produced by transformed E. ananas NR-1 was demonstrated in vitro by the inhibition of Pyricularia oryzae germ tube elongation such as bursting of the hyphal tip. Transformed E. ananas NR-1 suppressed the incidence of rice blast caused by P. oryzae under greenhouse conditions; however, the magnitude of the suppressive effect depended on which promoter was used. Both transformants and the nontransformant E. ananas NR-1 survived on rice phylloplane. It is expected that the rice epiphytic bacterium E. ananas NR-1 carrying a chitinolytic enzyme gene is an efficient biological control agent against rice blast. |
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Keywords: | Biological control Rice blast Chitinolytic enzyme gene Promoter Genetically modified microorganism |
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