| 影响刺梨花药愈伤组织诱导因素的研究 |
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| 引用本文： | 张绿萍,陈红. 影响刺梨花药愈伤组织诱导因素的研究[J]. 山地农业生物学报, 2009, 28(1) |
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| 作者姓名： | 张绿萍 陈红 |
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| 作者单位： | 1. 贵州大学,喀斯特山地果树资源研究所,贵州,贵阳,550025;贵州省果树研究所,贵州,罗甸,550100
2. 贵州大学,喀斯特山地果树资源研究所,贵州,贵阳,550025 |
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| 基金项目： | 贵州省科学技术基金，贵州省省长基金，贵州大学引进人才科研项目 |
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| 摘 要： | 以贵农1号、2号、5号、6号、7号刺梨为材料,研究不同基因型、不同取材时间、小孢子发育时期、基本培养基、糖源、生长调节物质、培养条件等刺梨花药愈伤组织诱导的影响.结果表明;以刺梨盛花期(5月份)且小孢子发育为单核中后期的贵农1号花药为试材;Ms为基本培养基;3%的蔗糖为糖源;1.0mg·L-1 6-BA +2.0mg·L-1 NAA +0.5mg·L-1 2、4-D +1.0mg·L-1 KT为激素组合,在黑暗条件下培养最有利于刺梨花药愈伤组织的诱导.
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| 关 键 词： | 刺梨 花药培养 愈伤组织诱导 |
Study of Influential factors on anther callus induction of Rosa roxbuighill Tratt |
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| ZHANG Lv-ping,CHEN Hong. Study of Influential factors on anther callus induction of Rosa roxbuighill Tratt[J]. Journal of Mountain Agriculture & Biology, 2009, 28(1) |
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| Authors: | ZHANG Lv-ping CHEN Hong |
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| Affiliation: | 1.Research Institute for Fruit Resources of Karst Mountain Region of Guizhou University;Guiyang Guizhou 550025;China;2.Research Institute for Fruit of Guizhou Province;Luodian Guizhou 550100;China |
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| Abstract: | To obtain anther callus of Rosa roxburghii Tratt,five genotypes including Guinong 1,Guinong 2,Guinong 5,Guinong 6,and Guinong 7 were employed to carry out various induction experiments in the present investigation.The results demonstrated that several factors,including genotype,developmental stage of microspore,basal medium,carbonic source,plant growth regulator(PGR),as well as incubating condition may have diverse effects on the callus formation.Anther of Guinong 1 obtained at full-blossom stage,whose deve... |
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| Keywords: | Rosa roxburghii Tratt Anther culture Callus induction |
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