克服昆明白小鼠胚胎发育阻滞的初步研究

为克服昆明白小鼠胚胎的2—细胞阻滞并使受精卵发育到囊胚期,本研究设两个实验组和一个对照组。第1实验组所用培养液为Brinster氏化学合成培养液与输卵管上皮和输卵管液混合的协同培养液;第2实验组用改良Brinster氏化学合成培养液;对照组用经典的Brinster培养液。培养是在5％CO_2和95％空气,37℃的CO_2箱内进行。3次体外培养试验结果是:第1实验组小鼠胚胎从受精卵发育到囊胚期平均发育率为43.7±7.76％;第2实验组的平均发育率为17.1±5.06％;对照组的平均发育率是15.3±3.06％。统计学分析表明,添加输卵管上皮和输卵管液的Brinster培养液对克服昆明白小鼠胚胎2—细胞阻滞具有一定作用。

PRIMARY STUDIES ON SURMOUNTING THE EMBRYONIC DEVELOPMENT BLOCK OF KUNMING WHITE MICE

In order to surmount the embryonic development block of Kunming white mice andmake the zygotes develop to the blastocysts stage, the animals were divided into two experi-mental groups and a control group. The medium of the first experimental group consisted ofBrinster's medium, oviduct epithelial cells and oviductal fluid. The medium of the secondgroup was modificatory Brinster's medium. The medium of the control group was Brinster'smedium. The embryos were cultured in a humidified atmosphere of 5% CO_2 and 95% air at 37℃. The experimental result was as follows: The average percentage of embryonic develop-ment from the zygotes to the blastocysts stage in the first group was 43.7±7.76%. In the sec-ond group it was 17.1±5.00%. In the control group it was 15.3±3.06%. The study testified that Brinster's medium with oviduct epithelial cells and oviductal fluidcould surmount the block of mouse embryonic development to a certain degree.